Insect vitellogenin (Vg) has been considered to be synthesized in the fat body. Here, we found that abundant Vg protein is synthesized in Laodelphax striatellus hemocytes as well. We also determined that only the hemocyte-produced Vg binds to Rice stripe virus (RSV) in vivo. Examination of the subunit composition of L. striatellus Vg (LsVg) revealed that LsVg was processed differently after its expression in different tissues. The LsVg subunit able to bind to RSV exist stably only in hemocytes, while fat body-produced LsVg lacks the RSV-interacting subunit. Nymph and male L. striatellus individuals also synthesize Vg but only in hemocytes, and the proteins co-localize with RSV. We observed that knockdown of LsVg transcripts by RNA interference decreased the RSV titer in the hemolymph, and thus interfered with systemic virus infection. Our results reveal the sex-independent expression and tissue-specific processing of LsVg and also unprecedentedly connect the function of this protein in mediating virus transmission to its particular molecular forms existing in tissues previously known as non-Vg producing.

昆虫卵黄蛋白原（Vg）被认为是在脂肪体内合成的。在这里，我们发现灰飞虱血细胞中也合成了丰富的Vg蛋白。我们还确定只有血细胞产生的 Vg 才能在体内与水稻条纹病毒 (RSV) 结合。检查L的亚基组成。灰飞虱Vg (LsVg)揭示了LsVg在不同组织中表达后的加工方式不同。能够与RSV结合的LsVg亚基仅稳定存在于血细胞中，而脂肪体产生的LsVg缺乏与RSV相互作用的亚基。若虫和雄性L .灰飞虱个体也合成 Vg，但仅在血细胞中，并且这些蛋白质与 RSV 共定位。我们观察到，通过 RNA 干扰敲除LsVg转录本会降低血淋巴中的 RSV 滴度，从而干扰全身病毒感染。我们的结果揭示了 LsVg 的性别依赖性表达和组织特异性加工，并且还前所未有地将这种蛋白质介导病毒传播的功能与其存在于先前称​​为非 Vg 产生的组织中的特定分子形式联系起来。