Neat Protein–Polymer Surfactant Bioconjugates as Universal Solvents,Biomacromolecules

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Neat Protein–Polymer Surfactant Bioconjugates as Universal Solvents
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-02-16 00:00:00 , DOI: 10.1021/acs.biomac.7b01729
Anasua Mukhopadhyay ₁ , Tarasankar Das ₁ , Anindya Datta ₁ , Kamendra P. Sharma ₁

Affiliation

Solvents, particularly those having low volatility, are of great interest for the biocatalytic synthesis of utility chemicals and fuels. We show novel and universal solvent-like properties of a neat and water-less polymer surfactant–bovine serum albumin (BSA) conjugated material (WL-PSpBSA). This highly viscous, nonvolatile material behaves as a liquid above its solid–liquid transition temperature (∼25–27 °C) and can be used as a solvent for variety of completely dry solutes of different sizes and surface chemistries. We show using a combination of optical microscopy and steady -state and time-resolved fluorescence spectroscopy that dry and powdered dyes (hydrophobic Coumarin 153 (C153)), enzymes (α-chymotrypsin (α-Chy)), or even 1 μm microparticles (diffusion coefficient ca. three orders slower than C153), can be solubilized and completely dispersed in the WL-PSpBSA solvent above 25 °C. While C153, irrespective of its mode of addition to WL-PSpBSA, binds similarly to BSA, α-Chy can be stabilized and activated to perform its hydrolysis function, even at 100 °C. This work therefore provides insights into the form of universal solvent characteristic property for this new class of nonaqueous, nonvolatile, biodegradable protein–polymer surfactant-based conjugated materials and suggests potential new avenues that can have a huge impact on biocatalysis, bionanotechnology, drug delivery, and other applications.

中文翻译：

天然蛋白质-聚合物表面活性剂生物共轭物作为通用溶剂

溶剂，特别是挥发性低的溶剂，对实用化学品和燃料的生物催化合成非常感兴趣。我们展示了一种纯净且无水的聚合物表面活性剂-牛血清白蛋白（BSA）共轭材料（WL-PS pBSA）。这种高粘度，不挥发的物质在高于其固液转变温度（约25–27°C）时表现为液体，可以用作各种尺寸和表面化学性质完全干燥的溶质的溶剂。我们展示了使用光学显微镜以及稳态和时间分辨荧光光谱技术的结合，可以干燥和粉化染料（疏水香豆素153（C153）），酶（α-胰凝乳蛋白酶（α-Chy））或什至1μm微粒（扩散系数比C153慢大约3个数量级），可以溶解并完全分散在25°C以上的WL-PS p BSA溶剂中。而C153，无论其添加到WL-PS p的方式如何BSA与BSA的结合方式相似，即使在100°C时，α-Chy也可以稳定并激活以执行其水解功能。因此，这项工作为这类新型非水，非挥发性，可生物降解的蛋白质-聚合物表面活性剂共轭材料提供了通用溶剂特性形式的见解，并提出了可能对生物催化，仿生技术，药物传递，和其他应用程序。

更新日期：2018-02-16

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