Growing evidence supports the importance of men's exposure to non-persistent endocrine disruptors (EDCs) and couple fecundability, as measured by time-to-pregnancy (TTP). This evolving literature contrasts with the largely equivocal findings reported for women's exposures and fecundity. While most evidence relies upon urinary concentrations, quantification of EDCs in seminal plasma may be more informative about potential toxicity arising within the testes. We analyzed 5 chemical classes of non-persistent EDCs in seminal plasma for 339 male partners of couples who were recruited prior to conception and who were followed daily until pregnant or after one year of trying. Benzophenones, bisphenols, parabens, and phthalate metabolites and phthalate diesters were measured using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) except for phthalate diesters, which were analyzed using gas chromatography-mass spectrometry. Cox regression with discrete-time was used to estimate fecundability odds ratios (FORs) and 95% confidence intervals (CIs) for each chemical to estimate the probability of pregnancy. While most EDCs were detected in seminal plasma, concentrations were lower than urinary concentrations previously analyzed for the cohort. None of the EDCs were significantly associated with fecundability even after covariate adjustment, though benzophenones consistently yielded FORs <1.0 (ranging from 0.72 to 0.91) in couple-adjusted models suggestive of diminished fecundity (longer TTP). The findings underscore that a range of EDCs can be quantified in seminal plasma, but the lower concentrations may require a large cohort for assessing couple fecundability, as well as the need to consider other fecundity outcomes such as semen quality.

越来越多的证据支持了男性接触非持续性内分泌干扰物（EDC）和夫妻生育能力的重要性，这可以通过怀孕时间（TTP）来衡量。不断发展的文献与有关妇女暴露和生殖力的报道大体上模棱两可的对比形成了鲜明的对比。尽管大多数证据都依赖于尿液浓度，但精液中EDC的定量可能对睾丸内产生的潜在毒性更为有益。我们分析了339对夫妻的男性伴侣在精浆中的5个化学类别的非持久性EDC，这些伴侣在受孕之前被招募，并且每天进行跟踪直至怀孕或尝试一年后。二苯甲酮，双酚，对羟基苯甲酸酯，除邻苯二甲酸二酯用气相色谱-质谱法分析外，其余均采用高效液相色谱-串联质谱法（HPLC-MS / MS）测定邻苯二甲酸酯的代谢产物和邻苯二甲酸二酯。使用离散时间的Cox回归来估计每种化学物质的受孕几率（FORs）和95％置信区间（CIs）以估计怀孕的可能性。虽然在精浆中检测到大多数EDC，但其浓度低于先前针对该人群进行分析的尿液浓度。即使在进行协变量调整后，EDC都没有与生育力显着相关，尽管二苯甲酮在夫妻调整模型中始终产生小于1.0的FORs（范围从0.72到0.91），提示生育力降低（TTP更长）。