Simultaneous response of fluorescence and light scattering can be obtained by using nanomaterials with size- and shape-dependent physiochemical properties. On the basis of this principle, we report a new strategy to design ratiometric sensors by combining fluorescence and light scattering, two different and independent signals. To obtain fluorescence and scattering signals simultaneously under the same excitation, two signal collection strategies are proposed based on the principles of fluorescence, light scattering and diffraction. One is to collect normal (down-conversion) fluorescence and second-order scattering (SOS) signals, and the other is to record the fluorescence excited by the second-order diffraction light of excitation wavelength λ/2 (SODL-fluorescence) and first-order scattering (FOS) or frequency doubling scattering (FDS) signals. A proof of concept study has been performed by using a carbon dots (CDs) and cobalt oxyhydroxide (CoOOH) nanoflakes system for ascorbic acid (AA) sensing. Apart from construction of ratiometric sensors, the combined fluorescence and scattering can also act as a useful technique to monitor aggregation-induced fluorescence quenching or enhancement.

中文翻译：

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荧光和光散射的尺寸依赖性调制：比率传感技术的发展新策略†

通过使用具有取决于大小和形状的物理化学特性的纳米材料，可以同时获得荧光和光散射的响应。基于此原理，我们报告了一种通过组合荧光和光散射，两个不同且独立的信号来设计比例传感器的新策略。为了在相同的激发下同时获得荧光和散射信号，基于荧光，光散射和衍射原理，提出了两种信号采集策略。一种是收集正常（下转换）荧光和二阶散射（SOS）信号，另一种是记录由激发波长为λ的二阶衍射光激发的荧光/ 2（SODL荧光）和一阶散射（FOS）或倍频散射（FDS）信号。通过使用碳点（CD）和羟基氧化钴（CoOOH）纳米薄片系统进行抗坏血酸（AA）感测，进行了概念验证研究。除了比例传感器的构造之外，荧光和散射相结合还可以用作监测聚集诱导的荧光猝灭或增强的有用技术。