Protein concentrates and isolates were developed from the microalga Spirulina sp. LEB 18 biomass and characterized by functional aspects for food application. Proteins were solubilized at pH 11 and precipitated at pH 4.2 (protein isoelectric point determined by potentiometric titration), with the aid of a high-speed homogenizer. With this procedure, it was possible to obtain a protein concentrate with 83.9 ± 1.7 wt% of protein and a protein isolate with 91.3 ± 1.2 wt%. Protein extraction from this microalga allowed a significant increase in protein solubility and foam stability. Furthermore, proteins from both concentrate and isolate presented higher resistance to thermal denaturation than the original proteins of Spirulina sp. LEB 18 biomass. These results show the application potential of the concentrate and the protein isolate from Spirulina sp. LEB 18 in foods for protein supplementation, since they have >80 and 90% protein, respectively.

Industrial relevance text

Using proteins from microalgal sources can serve as a sustainable alternative to meet world food demand. The isoelectric precipitation extraction method can be applied on a large scale, achieving high yields. Protein extracts present potential application in specific foods for protein supplementation. Protein extraction can be allied to other biocomposites extraction, such as carbohydrates and lipids for biofuels production.

中文翻译：

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微藻来源的高蛋白成分：获得和功能特性

蛋白质浓缩物和分离物是从微藻螺旋藻中开发的。LEB 18生物量，具有食品应用功能方面的特征。在高速匀浆器的帮助下，蛋白质在pH值为11的条件下溶解并在pH值为4.2的条件下沉淀（通过电位滴定法确定的蛋白质等电点）。通过该程序，可以得到具有83.9±1.7重量％的蛋白质的蛋白质浓缩物和具有91.3±1.2重量％的蛋白质分离物。从该微藻中提取蛋白质可以显着提高蛋白质的溶解度和泡沫稳定性。此外，浓缩物和分离物的蛋白质都比螺旋藻的原始蛋白质具有更高的抗热变性能力sp。LEB 18生物量。这些结果显示了螺旋藻浓缩物和分离蛋白的应用潜力。蛋白质补充食品中的LEB 18分别具有> 80％和90％的蛋白质。

行业相关文字

使用来自微藻源的蛋白质可以作为满足世界粮食需求的可持续选择。等电沉淀提取方法可以大规模应用，实现高产量。蛋白质提取物目前在特定食品中用于蛋白质补充的潜在应用。蛋白质提取可以与其他生物复合物提取相关，例如用于生物燃料生产的碳水化合物和脂质。