This study evaluated the ability of Brassica napus for extracting gold (Au), silver (Ag) and copper (Cu) from a mine tailings, with the inoculation of two Aspergillus niger strains, and the application of ammonium thiocyanate (NH₄SCN) or ammonium thiosulfate [(NH₄)₂S₂O₃]. After seven weeks of growth inoculated or non-inoculated plants were applied with 1 or 2 g kg⁻¹ of either NH₄SCN or (NH₄)₂S₂O₃, respectively. Eight days after the application of the chemical compounds, plants were harvested for determining the total dry biomass, and the content of Au, Ag, and Cu in plant organs. Application of (NH₄)₂S₂O₃ or NH₄SCN resulted in enhanced Au-accumulation in stems (447% and 507%, respectively), while either (NH₄)₂S₂O₃+Aspergillus, or NH₄SCN increased the Au-accumulation in roots (198.5% and 404%, respectively) when compared to the control. Treatments with (NH₄)₂S₂O₃ or (NH₄)₂S₂O₃+Aspergillus significantly increased (P ≤ 0.001) the accumulation of Ag in leaves (677% and 1376%, respectively), while NH₄SCN + Aspergillus, and (NH₄)₂S₂O₃ enhanced the accumulation in stems (7153% and 6717.5%). The Ag-accumulation in roots was stimulated by NH₄SCN+ Aspergillus, and (NH₄)₂S₂O₃+ Aspergillus (132.5% and 178%, respectively), when compared to the control. The combination of NH₄SCN+Aspergillus significantly enhanced the Cu-accumulation in leaves (228%); whereas NH₄SCN+ Aspergillus, or (NH₄)₂S₂O₃+ Aspergillus resulted in greater accumulation of Cu in stems (1233.5% and 1580%, respectively) than the control. Results suggest that either NH₄SCN or (NH₄)₂S₂O₃ (with or without Aspergillus) improved the accumulation of Au and Ag by B. napus. Accumulation of Au and Ag in plant organs overpassed the hyperaccumulation criterion (> 1 mg kg⁻¹ of plant biomass); whereas Cu-accumulation in stems and roots also overpassed such criterion (> 1000 mg kg⁻¹) by applying either NH₄SCN or (NH₄)₂S₂O₃ + A. niger.

这项研究评估了甘蓝型油菜通过接种两种黑曲霉菌株，从硫尾矿中提取金（Au），银（Ag）和铜（Cu）的能力以及硫氰酸铵（NH ₄ SCN）的应用。硫代硫酸铵[（NH ₄）₂ S ₂ O ₃ ]。生长七个星期后，向接种或未接种的植物施用1或2 g kg ^-1的NH ₄ SCN或（NH ₄）₂ S ₂ O ₃， 分别。施用化合物八天后，收获植物以确定总干生物量以及植物器官中Au，Ag和Cu的含量。（NH ₄）₂ S ₂ O ₃或NH ₄ SCN的施用增加了茎中的Au积累（分别为447％和507％），而（NH ₄）₂ S ₂ O ₃ +曲霉或NH ₄与对照相比，SCN增加了根部的Au积累（分别为198.5％和404％）。用（NH ₄）₂ S ₂ O处理₃或（NH ₄）₂ S ₂ O ₃ +曲霉显着增加（P≤0.001）叶片中银的积累（分别为677％和1376％），而NH ₄ SCN + 曲霉和（NH ₄）₂ S ₂ O ₃增强茎中的积累（7153％和6717.5％）。NH ₄ SCN + 曲霉菌和（NH ₄）₂ S ₂ O ₃ + 曲霉菌能刺激根部积累银。（分别为132.5％和178％）。NH ₄ SCN +曲霉菌的组合显着增强了叶片中铜的积累（228％）；而NH ₄ SCN + 曲霉或（NH ₄）₂ S ₂ O ₃ + 曲霉会导致铜在茎中的积累量更大（分别为1233.5％和1580％）。结果表明，无论是NH ₄ SCN还是（NH ₄）₂ S ₂ O ₃（有或没有曲霉菌），均可通过以下方式提高Au和Ag的积累：甘蓝型油菜。植物器官中金和银的积累超过了超积累标准（> 1 mg kg ^-1的植物生物量）；而通过施用NH ₄ SCN或（NH ₄）₂ S ₂ O ₃ + 黑曲霉，茎和根中的铜积累也超过了该标准（> 1000 mg kg ^-1）。