Marine and estuarine macroalgae are increasingly used for direct consumption, due to its high content of essential nutrients. However, the toxicity of chemical forms of arsenic (As) present in macroalgae are becoming of great concern and an important research topic.

This review will discuss the sophisticated analytical methodologies used for arsenic speciation, as well the investigation of cheaper and fit for purpose methods able to inform about the safety of macroalgae consumption. Furthermore, we will also discuss relevant speciation studies performed by various authors highlighting the methodologies and respective figures of merit for all components of As in macroalgae, not only inorganic As (iAs). Focusing on the steps prior to speciation, such as sample extraction and clean-up, it will be stressed the need to develop certified reference materials (CRMs) in order to assist in the validation of analytical methodologies for As speciation in macroalgae.

海洋和河口大型藻类由于其必需营养素含量高而越来越多地用于直接消费。然而，存在于大型藻类中的化学形式的砷（As）的毒性正在引起人们的广泛关注和重要的研究课题。

这篇综述将讨论用于砷形态分析的复杂分析方法，以及对能够告知大型藻类消费安全性的更便宜且适合特定用途的方法的研究。此外，我们还将讨论由不同作者进行的有关物种形成的研究，重点介绍大型藻类中As的所有组分的方法和各自的优值，而不仅仅是无机As（iAs）。着重于物种形成之前的步骤，例如样品提取和净化，将强调需要开发经认证的参考物质（CRM），以帮助验证大型藻类中砷物种形成的分析方法。