In this research, we have designed, fabricated and characterized an electrical double layer (EDL)-gated AlGaN/GaN high electron mobility transistor (HEMT) biosensor array to study the transmembrane potential changes of cells. The sensor array platform is designed to detect and count circulating tumor cells (CTCs) of colorectal cancer (CRC) and investigate cellular bioelectric signals. Using the EDL FET biosensor platform, cellular responses can be studied in physiological salt concentrations, thereby eliminating complex automation. Upon investigation, we discovered that our sensor response follows the transmembrane potential changes of captured cells. Our whole cell sensor platform can be used to monitor the dynamic changes in the membrane potential of cells. The effects of continuously changing electrolyte ion concentrations and ion channel blocking using cadmium are investigated. This methodology has the potential to be used as an electrophysiological probe for studying ion channel gating and the interaction of biomolecules in cells. The sensor can also be a point-of-care diagnostic tool for rapid screening of diseases.

中文翻译：

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动态监测跨膜电位变化：使用双层门控FET生物传感器对离子通道进行研究†

在这项研究中，我们设计，制造和表征了双电层（EDL）门控的AlGaN / GaN高电子迁移率晶体管（HEMT）生物传感器阵列，以研究细胞的跨膜电位变化。传感器阵列平台旨在检测和计数结直肠癌（CRC）的循环肿瘤细胞（CTC）并研究细胞生物电信号。使用EDL FET生物传感器平台，可以在生理盐浓度下研究细胞反应，从而消除了复杂的自动化过程。经调查，我们发现我们的传感器响应遵循捕获的细胞的跨膜电位变化。我们的整个细胞传感器平台可用于监测细胞膜电位的动态变化。研究了使用镉不断变化的电解质离子浓度和离子通道阻塞的影响。这种方法学有可能被用作研究细胞中离子通道门控和生物分子相互作用的电生理探针。该传感器还可以是即时诊断疾病的即时诊断工具。