Pervasive, Coordinated Protein-Level Changes Driven by Transcript Isoform Switching during Meiosis.,Cell

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Pervasive, Coordinated Protein-Level Changes Driven by Transcript Isoform Switching during Meiosis.
Cell ( IF 45.5 ) Pub Date : 2018-Feb-22 , DOI: 10.1016/j.cell.2018.01.035
Ze Cheng ₁ , George Maxwell Otto ₁ , Emily Nicole Powers ₁ , Abdurrahman Keskin ₂ , Philipp Mertins ₃ , Steven Alfred Carr ₃ , Marko Jovanovic ₂ , Gloria Ann Brar ₁

Affiliation

To better understand the gene regulatory mechanisms that program developmental processes, we carried out simultaneous genome-wide measurements of mRNA, translation, and protein through meiotic differentiation in budding yeast. Surprisingly, we observed that the levels of several hundred mRNAs are anti-correlated with their corresponding protein products. We show that rather than arising from canonical forms of gene regulatory control, the regulation of at least 380 such cases, or over 8% of all measured genes, involves temporally regulated switching between production of a canonical, translatable transcript and a 5' extended isoform that is not efficiently translated into protein. By this pervasive mechanism for the modulation of protein levels through a natural developmental program, a single transcription factor can coordinately activate and repress protein synthesis for distinct sets of genes. The distinction is not based on whether or not an mRNA is induced but rather on the type of transcript produced.

中文翻译：

减数分裂过程中由转录异构体转换驱动的普遍，协调的蛋白质水平变化。

为了更好地了解对发育过程进行编程的基因调控机制，我们通过发芽酵母中的减数分裂进行了全基因组范围内的mRNA，翻译和蛋白质的同时测量。令人惊讶地，我们观察到数百个mRNA的水平与其相应的蛋白质产物抗相关。我们表明，不是由规范形式的基因调节控制引起的，至少有380个此类病例或占所有已测基因的8％的调节涉及在规范的，可翻译的转录本和5'延伸的同工型之间产生时间调控不能有效地翻译成蛋白质。通过这种通过自然发育程序调节蛋白质水平的普遍机制，单个转录因子可以协调激活并抑制不同基因组的蛋白质合成。区别不是基于是否诱导mRNA，而是基于产生的转录本的类型。

更新日期：2018-02-22

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