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Mating-Induced Differential Peptidomics of Neuropeptides and Protein Hormones in Agrotis ipsilon Moths
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-02-28 00:00:00 , DOI: 10.1021/acs.jproteome.7b00779 Max Diesner 1 , Aurore Gallot 2 , Hellena Binz 3 , Cyril Gaertner 2 , Simon Vitecek 2 , Jörg Kahnt 4 , Joachim Schachtner 1 , Emmanuelle Jacquin-Joly 2 , Christophe Gadenne 5
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-02-28 00:00:00 , DOI: 10.1021/acs.jproteome.7b00779 Max Diesner 1 , Aurore Gallot 2 , Hellena Binz 3 , Cyril Gaertner 2 , Simon Vitecek 2 , Jörg Kahnt 4 , Joachim Schachtner 1 , Emmanuelle Jacquin-Joly 2 , Christophe Gadenne 5
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In many insects, mating induces drastic changes in male and female responses to sex pheromones or host-plant odors. In the male moth Agrotis ipsilon, mating induces a transient inhibition of behavioral and neuronal responses to the female sex pheromone. As neuropeptides and peptide hormones regulate most behavioral processes, we hypothesize that they could be involved in this mating-dependent olfactory plasticity. Here we used next-generation RNA sequencing and a combination of liquid chromatography, matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and direct tissue profiling to analyze the transcriptome and peptidome of different brain compartments in virgin and mated males and females of A. ipsilon. We identified 37 transcripts encoding putative neuropeptide precursors and 54 putative bioactive neuropeptides from 23 neuropeptide precursors (70 sequences in total, 25 neuropeptide precursors) in different areas of the central nervous system including the antennal lobes, the gnathal ganglion, and the corpora cardiaca–corpora allata complex. Comparisons between virgin and mated males and females revealed tissue-specific differences in peptide composition between sexes and according to physiological state. Mated males showed postmating differences in neuropeptide occurrence, which could participate in the mating-induced olfactory plasticity.
中文翻译：
冬虫夏草蛾中神经肽和蛋白质激素的交配诱导差异化肽组学
在许多昆虫中，交配引起雄性和雌性对性信息素或寄主植物气味的反应急剧变化。在雄蛾Agrotis ipsilon中，交配会引起对雌性信息素的行为和神经元反应的短暂抑制。由于神经肽和肽激素调节大多数行为过程，我们假设它们可能参与这种依赖交配的嗅觉可塑性。在这里，我们使用了下一代RNA测序以及液相色谱，基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱和直接组织分析的组合，以分析原始和不同脑室中的转录组和肽组。交配的A. ipsilon的雄性和雌性。我们从中枢神经系统的不同区域（包括触角，叶神经节，cardiac神经节和cardiac体）中的23种神经肽前体（总共70个序列，25种神经肽前体）中鉴定出37种编码假定的神经肽前体和54种假定的生物活性神经肽。阿拉塔复合体。处女和交配的雄性和雌性之间的比较揭示了性别之间和根据生理状态的肽组成的组织特异性差异。交配的雄性显示出神经肽发生后的差异，这可能参与交配诱导的嗅觉可塑性。
更新日期：2018-03-01
中文翻译：
冬虫夏草蛾中神经肽和蛋白质激素的交配诱导差异化肽组学
在许多昆虫中，交配引起雄性和雌性对性信息素或寄主植物气味的反应急剧变化。在雄蛾Agrotis ipsilon中，交配会引起对雌性信息素的行为和神经元反应的短暂抑制。由于神经肽和肽激素调节大多数行为过程，我们假设它们可能参与这种依赖交配的嗅觉可塑性。在这里，我们使用了下一代RNA测序以及液相色谱，基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱和直接组织分析的组合，以分析原始和不同脑室中的转录组和肽组。交配的A. ipsilon的雄性和雌性。我们从中枢神经系统的不同区域（包括触角，叶神经节，cardiac神经节和cardiac体）中的23种神经肽前体（总共70个序列，25种神经肽前体）中鉴定出37种编码假定的神经肽前体和54种假定的生物活性神经肽。阿拉塔复合体。处女和交配的雄性和雌性之间的比较揭示了性别之间和根据生理状态的肽组成的组织特异性差异。交配的雄性显示出神经肽发生后的差异，这可能参与交配诱导的嗅觉可塑性。
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