A combination between modification with porous layer and grafting of polyethyleneimine (PEI) on the inner face of capillary was for the first time developed for boronate affinity in-tube solid-phase microextraction (SPME) material to enhance the extraction capacity for cis-diol-containing polyphenols. The successful synthesis of boronate-decorated polyethyleneimine-grafted porous layer open tubular (BPPLOT) capillary was confirmed by scanning electron micrograph, Fourier transform-infrared spectra and absorption experiments. The porous layer, PEI and boronate affinity provided high specific surface area, more binding sites for boronate groups and specific selectivity of BPPLOT capillary, respectively. The maximum binding quantity of BPPLOT capillary greatly improved, and ranged from 143 to 170 μg m⁻¹ for cis-diol-containing polyphenols (catechin, chlorogenic acid, caffeic acid and epicatechin). A green method based on boronate affinity in-tube SPME was developed for separation and enrichment polyphenols, and some parameters of in-tube SPME were optimized. After in-tube SPME, HPLC with UV detection was used for quantitative determination of polyphenols. Recoveries of standard spiked cis-diol-containing polyphenols from fruit juice were between 80.9% and 102%, with intra-day and inter-day coefficient of variation ranging from 4.8% to 7.3% and 5.0% to 8.6%, respectively. Conversely, recovery of non-cis-diol-containing ferulic acid was no greater than 3.0%. These results suggested that the BPPLOT capillary could effectively separate and enrich cis-diol-containing polyphenols from real samples.

首次开发了多孔层修饰与在毛细管内表面接枝聚乙烯亚胺（PEI）之间的组合，以用于硼酸酯亲和管内固相微萃取（SPME）材料，以增强对顺式-二醇-的萃取能力含有多酚。通过扫描电子显微镜，傅立叶变换红外光谱和吸收实验证实了硼酸酯修饰的聚乙烯亚胺接枝多孔层开管（BPPLOT）毛细管的成功合成。多孔层，PEI和硼酸酯亲和力分别提供了高比表面积，更多的硼酸酯基结合位点和BPPLOT毛细管的比选择性。BPPLOT毛细管的最大结合量大大提高，范围为143至170μgm^-1为含顺式二醇的多酚（儿茶素，绿原酸，咖啡酸和表儿茶素）。提出了一种基于硼酸酯亲和管内SPME的绿色分离和富集多酚的方法，并优化了管内SPME的一些参数。管内SPME后，使用带有UV检测的HPLC定量测定多酚。从果汁中回收的标准加标含顺式二醇的多酚含量在80.9％至102％之间，日内和日间变异系数分别为4.8％至7.3％和5.0％至8.6％。相反，恢复非顺式含-二醇的阿魏酸不大于3.0％。这些结果表明，BPPLOT毛细管可以有效地从真实样品中分离和富集含顺式二醇的多酚。