Oral cholera vaccination is used to induce immune responses in the intestines to protect against cholera infection. However, oral vaccination may also affect immune responses in other mucosal tissues. To study this, tissue-specific homing potential and kinetics of B-cell responses were characterized after oral cholera vaccination. Healthy adult volunteers received two doses of Dukoral® and blood, saliva, nasal wash, and fecal samples were collected over time to detect vaccine-specific antibodies. Additionally, homing potential of lymphocytes to small intestine, colon, airways, skin, and periphery was measured by expression of Integrin β1 and β7, CCR9, CCR10, CCR7, and CLA. After vaccination, antibody responses to cholera toxin B (CTB) and Dukoral® were detected in serum and nasal wash. CTB-specific memory B cells in peripheral blood and tissue homing profiles of memory B cells peaked at day 18. IgA⁺ memory B cells expressed markers that enable homing to the airways and colon, while IgA^- memory B cells primarily expressed small-intestine-homing markers. These data show that oral cholera vaccination has a differential effect on immune responses in various mucosal sites, including the respiratory tract.

中文翻译：

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口服霍乱疫苗可促进 IgA+ 记忆 B 细胞归巢至大肠和呼吸道。

口服霍乱疫苗用于在肠道中诱导免疫反应，以防止霍乱感染。然而，口服疫苗接种也可能影响其他粘膜组织的免疫反应。为了研究这一点，在口服霍乱疫苗后表征了组织特异性归巢潜力和 B 细胞反应动力学。健康的成年志愿者接受了两剂 Dukoral®，并随着时间的推移收集了血液、唾液、鼻腔清洗液和粪便样本，以检测疫苗特异性抗体。此外，通过整合素 β1 和 β7、CCR9、CCR10、CCR7 和 CLA 的表达来测量淋巴细胞向小肠、结肠、气道、皮肤和外周的归巢潜力。接种疫苗后，在血清和鼻洗液中检测到对霍乱毒素 B (CTB) 和 Dukoral® 的抗体反应。⁺记忆 B 细胞表达能够归巢至气道和结肠的标记，而 IgA ^-记忆 B 细胞主要表达小肠归巢标记。这些数据表明，口服霍乱疫苗对不同粘膜部位（包括呼吸道）的免疫反应具有不同的影响。