Se-Enriched defatted rapeseed (Se-DRS), a by-product of rapeseed oil production, is high in Se. This study aimed to detect possible changes in Se-metabolism after Se-DRS consumption and to assess its suitability as a Se supplement in monogaster feed. In this experiment, rats were fed three types of diets differing in Se-form: soybean meal, Se-salt and Se-DRS. Potential differences in Se-metabolism were evaluated based on the total Se concentrations along with non-protein Se speciation patterns in the urine and blood serum of the rats. The total Se concentrations were determined using inductively coupled plasma-mass spectrometry (ICP-MS). Se compounds in the samples were identified through high-performance liquid chromatography/ICP-MS. The total Se excretion rate was dose-dependent, and excess of dietary Se was eliminated in the form of Se-sugar 1, regardless of the administered Se-form. The excretion dynamics of dietary selenomethionine (soybean meal) and methylselenocysteine (Se-DRS) were different to that of the mineral Se (Se-salt). The proportions of Se species in the urine were significantly influenced by the Se source. A specific metabolic pathway, resulting in urinary excretion of Se-sugar 2, was observed in animals fed Se-DRS. Neither the total Se concentrations nor the serum Se speciation patterns differed between the animals fed the conventional diet and those fed the Se-DRS-enriched diet. Based on these results, Se-DRS can be considered a suitable Se supplement for monogaster nutrition in Se-deficient regions.

中文翻译：

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饲喂富含硒的脱脂菜籽（甘蓝型油菜）饮食的大鼠中硒的硒的代谢转化和尿排泄†

富硒的脱脂菜籽（Se-DRS）是菜籽油生产的副产品，含硒量很高。这项研究旨在检测食用Se-DRS后Se代谢的可能变化，并评估其在Monogaster饲料中作为Se补充剂的适用性。在该实验中，给大鼠喂食了三种硒形式不同的饮食：豆粕，硒盐和硒-DRS。根据总硒浓度以及大鼠尿液和血清中的非蛋白质硒形态模式，评估硒代谢的潜在差异。使用电感耦合等离子体质谱法（ICP-MS）确定总Se浓度。样品中的硒化合物通过高效液相色谱/ ICP-MS鉴定。硒的总排泄率是剂量依赖性的，以硒糖1的形式消除了饮食中过量的硒。不论施用的Se形式如何。日粮硒代蛋氨酸（大豆粉）和甲基硒代半胱氨酸（Se-DRS）的排泄动态与矿物质硒（Se-盐）的排泄动态不同。尿中硒种类的比例受硒源的影响很大。在饲喂Se-DRS的动物中观察到一种特定的代谢途径，导致尿糖2排泄。在饲喂常规饮食的动物和饲喂富含Se-DRS的饮食的动物之间，总硒浓度和血清硒形态均无差异。根据这些结果，可以将Se-DRS视为缺硒地区Monogaster营养的合适Se补充剂。日粮硒代蛋氨酸（大豆粉）和甲基硒代半胱氨酸（Se-DRS）的排泄动态与矿物质硒（Se-盐）的排泄动态不同。尿中硒种类的比例受硒源的影响很大。在饲喂Se-DRS的动物中观察到一种特定的代谢途径，导致尿糖2排泄。在饲喂常规饮食的动物和饲喂富含Se-DRS的饮食的动物之间，总硒浓度和血清硒形态均无差异。根据这些结果，可以将Se-DRS视为缺硒地区Monogaster营养的合适Se补充剂。日粮硒代蛋氨酸（大豆粉）和甲基硒代半胱氨酸（Se-DRS）的排泄动态与矿物质硒（Se-盐）的排泄动态不同。尿中硒种类的比例受硒源的影响很大。在饲喂Se-DRS的动物中观察到一种特定的代谢途径，导致尿中糖2的排泄。在饲喂常规饮食的动物和饲喂富含Se-DRS的饮食的动物之间，总硒浓度和血清硒形态均无差异。根据这些结果，可以将Se-DRS视为缺硒地区Monogaster营养的合适Se补充剂。在饲喂Se-DRS的动物中观察到了这种现象。在饲喂常规饮食的动物和饲喂富含Se-DRS的饮食的动物之间，总硒浓度和血清硒形态均无差异。根据这些结果，可以将Se-DRS视为缺硒地区Monogaster营养的合适Se补充剂。在饲喂Se-DRS的动物中观察到了这种现象。在饲喂常规饮食的动物和饲喂富含Se-DRS的饮食的动物之间，总硒浓度和血清硒形态均无差异。根据这些结果，可以将Se-DRS视为缺硒地区Monogaster营养的合适Se补充剂。