Reconstitution of composite actin and keratin networks in vesicles,Soft Matter

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Reconstitution of composite actin and keratin networks in vesicles
Soft Matter ( IF 2.9 ) Pub Date : 2018-02-21 00:00:00 , DOI: 10.1039/c7sm00819h
J. Deek _{1,

2,

3} , R. Maan _{1,

2,

3} , E. Loiseau _{1,

2,

3} , A. R. Bausch _{1,

2,

3}

Affiliation

Although cytoskeletal networks are interpenetrating and interacting in living cells, very little is understood as to the effect their interaction has on their properties. Here, as a step towards elucidating the synergistic cellular role of these structural proteins, we investigate isolated keratin and actin composites and show how the in vitro network formation of keratin influences the properties of actin networks and vice versa. By encapsulating purified composite networks into vesicles and separating the time scales of network formation we are able to demonstrate that the actin network stabilizes keratin networks by providing an elastic resistance to their collapse in vitro.

中文翻译：

囊泡中复合肌动蛋白和角蛋白网络的重构

尽管细胞骨架网络在活细胞中相互渗透和相互作用，但对其相互作用对其性能的影响了解甚少。在这里，作为阐明这些结构蛋白的协同细胞作用的一步，我们研究了分离的角蛋白和肌动蛋白复合物，并显示了角蛋白的体外网络形成如何影响肌动蛋白网络的特性，反之亦然。通过将纯化的复合网络封装到囊泡中，并分离网络形成的时间尺度，我们能够证明肌动蛋白网络通过对体外塌陷提供弹性抵抗来稳定角蛋白网络。

更新日期：2018-02-21

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