A facile phytosynthesis of Ag/AgCl and Ag nanoparticles (NPs) was developed using a crude water extract of Citrus hystrix DC (kaffir lime) leaves. The phytochemical and chloride contents of the extract, and various synthesis parameters were studied. The obtained Ag/AgCl and Ag NPs were of the cubic phase and mostly spherical in shape. Particle sizes of the yielded NPs were distributed in a narrow range with an average size of 20 and 38 nm for the Ag/AgCl and Ag NPs, respectively. The reducing function of the phytochemicals and the stabilizing ability of gelatin were demonstrated. The NPs showed significant anti-proliferative activities against the carcinoma HCT 116 and adenocarcinoma Caco-2 colorectal cell lines, but had a negative effect toward human fibroblasts. The NPs exhibited excellent antibacterial activities against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus cereus. The inactivation kinetics obtained in the presence of the NPs showed a biphasic behavior that can be explained by the Cerf model.

利用柑桔的粗提物开发了一种易于进行的Ag / AgCl和Ag纳米颗粒（NPs）的植物合成。DC（非洲柠檬）叶子。研究了提取物的植物化学和氯化物含量，以及各种合成参数。所获得的Ag / AgCl和Ag NP为立方相，形状大多为球形。对于Ag / AgCl和Ag NPs，所产生的NP的粒径分布在窄范围内，平均尺寸分​​别为20和38nm。证明了植物化学物质的还原功能和明胶的稳定能力。NPs对HCT 116癌和Caco-2结肠癌腺癌细胞具有显着的抗增殖活性，但对人成纤维细胞有不利影响。NPs对大肠杆菌，金黄色葡萄球菌，铜绿假单胞菌表现出优异的抗菌活性。和蜡状芽孢杆菌。在NPs存在下获得的失活动力学表现出双相行为，这可以通过Cerf模型来解释。