Abstract This work presents the development and application of a biosensor based on cadmium selenide/zinc sulphide core-shell quantum dot modified with the redox protein cytochrome c (CdSe/ZnS-Cytc) for the photoelectrochemical determination of epinephrine (EP). The photoactive biocomposite was immobilized on the surface of a glass substrate modified with indium doped tin oxide (CdSe/ZnS-Cytc/ITO). The photoactive film was characterized by electrochemical impedance spectroscopy and the photocurrent measurements were obtained by chronoamperometry using a LED light lamp as source of irradiation. After the experimental parameters optimization, the biosensor presented a good response for EP oxidation in a wide linear concentration range between 1 μmol L−1 and 1.2 mmol L−1 (with r = 0.999; n = 9). A detection limit of 2 nmol L−1 was obtained. The selectivity of the photosensor was evaluated against species such as uric acid, ascorbic acid, folic acid, barbital, glucose, and urea, and the results showed that the proposed biosensor has a good selectivity for the detection of EP. The CdSe/ZnS-Cytc/ITO photoelectrochemical biosensor was applied in injectable drug samples used in cardiopulmonary resuscitation. The recovery tests in the samples showed recovery values between 101 and 110% suggesting a good accuracy for the proposed method.

中文翻译：

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利用细胞色素 c 修饰的 CdSe/ZnS 核壳光催化剂测定心肺复苏药物中的肾上腺素

摘要 这项工作介绍了基于硒化镉/硫化锌核壳量子点的生物传感器的开发和应用，该生物传感器用氧化还原蛋白细胞色素 c (CdSe/ZnS-Cytc) 修饰，用于肾上腺素 (EP) 的光电化学测定。将光活性生物复合材料固定在用铟掺杂的氧化锡（CdSe/ZnS-Cytc/ITO）改性的玻璃基材的表面上。光敏膜通过电化学阻抗谱表征并且光电流测量通过使用LED灯作为照射源的计时电流法获得。在实验参数优化后，生物传感器在 1 μmol L-1 和 1.2 mmol L-1 之间的宽线性浓度范围内对 EP 氧化表现出良好的响应（r = 0.999；n = 9）。获得了 2 nmol L-1 的检测限。评估了光电传感器对尿酸、抗坏血酸、叶酸、巴比妥、葡萄糖和尿素等物种的选择性，结果表明所提出的生物传感器对 EP 的检测具有良好的选择性。CdSe/ZnS-Cytc/ITO光电化学生物传感器应用于心肺复苏注射药物样品。样品中的回收率测试显示回收率值介于 101% 和 110% 之间，表明所提出的方法具有良好的准确性。