Abstract In this study, gold nanostructures (AuNSs) and silver nanoparticles (AgNPs) were integrated with a silver micro-flower-like structure deposited on a screen-printed carbon electrode (AgMF-SPCE) for enhancing surface-enhanced Raman scattering (SERS) by using 4-mercaptobenzoic acid (4-MBA) as a Raman reporter. SERS was enhanced by approximately 3.6–52.1-fold, depending on the frequency of the incident laser, the localized surface plasmon resonance frequency of metallic NPs, and particle–particle aggregation effects. Compared with AgNP/SPCE and AgMF-SPCE substrates, the AgNP/AgMF-SPCE substrate showed high temperature tolerance and long-term durability. Furthermore, the proposed substrates easily obtained hot spots for other Raman reporters such as 4-aminothiophenol, 5,5′-dithiobis-2-nitrobenzoic acid, and 4-chlorothiophenol. A linear relationship was found between the Raman signal and the concentration of Raman reporters in the range 10 nM–100 μM, with the limit of detection in the range of 6.19–77.2 nM at a signal-to-noise ratio of 3.0. These results suggest that the AgNP/AgMF-SPCE substrate will be well suited for quantitative analysis.

中文翻译：

---

由于金属纳米粒子和基底之间的局部表面等离子体共振耦合，表面增强拉曼散射增强

摘要 在这项研究中，金纳米结构 (AuNSs) 和银纳米颗粒 (AgNPs) 与沉积在丝网印刷碳电极 (AgMF-SPCE) 上的银微花状结构相结合，以增强表面增强拉曼散射 (SERS)通过使用 4-巯基苯甲酸 (4-MBA) 作为拉曼报告分子。SERS 增强了大约 3.6-52.1 倍，这取决于入射激光的频率、金属纳米粒子的局部表面等离子体共振频率以及粒子-粒子聚集效应。与 AgNP/SPCE 和 AgMF-SPCE 基板相比，AgNP/AgMF-SPCE 基板表现出耐高温和长期耐用性。此外，所提出的底物很容易获得其他拉曼报告分子的热点，如 4-氨基苯硫酚、5,5'-二硫代双-2-硝基苯甲酸和 4-氯苯硫酚。在 10 nM–100 μM 范围内发现拉曼信号与拉曼报告基因浓度之间存在线性关系，检测限在 6.19–77.2 nM 范围内，信噪比为 3.0。这些结果表明 AgNP/AgMF-SPCE 底物将非常适合定量分析。