A novel kind of hybrid monolith with well-defined mesopore structure was prepared based on sol-gel condensation of 1, 2-bis(trimethoxysilyl)ethane (BTME) and tetramethoxysilane. Compared with terminal organosiloxanes used for preparation of conventional hybrid monoliths, BTME, as an ethane-bridged alkoxysilane precursor, could not only maintain the uniformity of pore size distribution, but also improve the chemical stability of the monolith via Si-C bonds in the framework. Owing to the controllable mesoporous structure and good stability, the monolithic column was used for the separation of peptides with half peak width less than 6 s and the run-to-run and column-to-column relative standard deviations (RSD) for the retention time of five standard peptides less than 2.5%, showing narrow peak width and good reproducibility. Moreover, the separation performance could be well maintained even after washed by the mobile phase with pH 11.0 at 50 °C. Furthermore, 100 cm-length monolithic column was prepared and successfully used for nanoRPLC-ESI-MS/MS analysis of HeLa cell lysate digests, and 5670 proteins corresponding to 37574 peptides were identified from 750 ng of the sample, showing great promising of "single-shot" large-scale in-depth proteomic research.

中文翻译：

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具有明确介孔性和高稳定性的乙烷桥连杂化整料，用于高性能肽分离

基于 1, 2-双（三甲氧基甲硅烷基）乙烷 (BTME) 和四甲氧基硅烷的溶胶-凝胶缩合，制备了一种具有明确介孔结构的新型杂化整料。与用于制备常规杂化整料的末端有机硅氧烷相比，BTME 作为乙烷桥连烷氧基硅烷前驱体，不仅可以保持孔径分布的均匀性，而且通过骨架中的 Si-C 键提高了整料的化学稳定性. 由于介孔结构可控且稳定性好，整体柱用于分离半峰宽小于6 s的肽段，以及保留的运行间和柱间相对标准偏差（RSD） 5 个标准肽的时间小于 2.5%，显示出窄峰宽和良好的重现性。而且，即使在 50 °C 下用 pH 11.0 的流动相洗涤后，分离性能仍能保持良好。此外，制备了100 cm长的整体柱并成功用于HeLa细胞裂解物消化物的nanoRPLC-ESI-MS/MS分析，从750 ng样品中鉴定出对应于37574个肽段的5670个蛋白质，显示出“单-shot”大规模深入的蛋白质组学研究。