Hypochlorous acid (HOCl), as an important reactive oxygen species (ROS), is involved with many pathological and physiological pathways, whereas many aspects of its roles remain unclear due to the lack of robust analytical methods. In this work, we report a novel kind of special reaction-based nanosensors, gold nanoparticles modified with newly synthesized 2-mercapto-4-methoxy-phenol molecules (AuNPs/MMP), to detect the endogenous HOCl in living cells according to the changes in the SERS spectrum of AuNPs/MMP resulting from the reaction of HOCl with MMP on AuNPs. These nanosensors can rapidly respond to HOCl within 1 min with the detection limit at 10-7 M level. Furthermore, the nanosensors exhibit high selectivity for HOCl because of the specificity of the reaction combined with the fingerprinting capability of SERS. Consequently, the proposed SERS nanosensors are capable of monitoring and imaging the endogenous HOCl generated in a living macrophage under stimulation, showing great promise to be used for in-situ studies of the pathophysiological processes that involve HOCl.

中文翻译：

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基于反应的 SERS 纳米传感器用于监测和成像活细胞中的内源性次氯酸

次氯酸 (HOCl) 作为一种重要的活性氧 (ROS)，涉及许多病理和生理途径，但由于缺乏可靠的分析方法，其作用的许多方面仍不清楚。在这项工作中，我们报告了一种新型的基于特殊反应的纳米传感器，用新合成的 2-巯基-4-甲氧基苯酚分子 (AuNPs/MMP) 修饰的金纳米粒子，根据变化检测活细胞中的内源性 HOCl在 AuNPs/MMP 的 SERS 光谱中，HOCl 与 MMP 在 AuNPs 上反应。这些纳米传感器可以在 1 分钟内快速响应 HOCl，检测限在 10-7 M 水平。此外，由于反应的特异性与 SERS 的指纹识别能力相结合，纳米传感器对 HOCl 表现出高选择性。最后，