Brentuximab vedotin (Adcetris) is a cysteine-linked antibody-drug conjugate (ADC) used in the treatment of Hodgkin lymphoma (HL) and systemic anaplastic large cell lymphoma (ALCL). In this study, the drug payload and glycan modifications of this ADC were simultaneously characterized using a unique LC-MS middle-up analysis, involving hydrophilic interaction chromatography (HILIC). This work demonstrates that HILIC is an effective and complementary analytical technique to reversed phase liquid chromatography (RPLC) for subunit-level characterization of immuno-conjugates.

中文翻译：

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通过亲水相互作用色谱-质谱联用对抗体-药物偶联物进行表征

Brentuximab vedotin（Adcetris）是半胱氨酸连接的抗体-药物偶联物（ADC），用于治疗霍奇金淋巴瘤（HL）和系统间变性大细胞淋巴瘤（ALCL）。在这项研究中，使用独特的LC-MS中上分析（包括亲水相互作用色谱法（HILIC））同时表征了该ADC的药物有效载荷和聚糖修饰。这项工作表明，HILIC是反相液相色谱（RPLC）的有效和互补的分析技术，用于免疫偶联物的亚基水平表征。