In this work, metal-organic framework particles incorporated fibers (UiO-66/PAN nanofibers) were used as adsorbent in pipette tip solid phase extraction (PT-SPE) for the first time. The UiO-66/PAN nanofibers were fabricated by a facile electrospinning method and characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and nitrogen adsorption-desorption experiments. The UiO-66/PAN nanofibers were applied to assemble a novel PT-SPE cartridge for determination of four phytohormones followed by high performance liquid chromatography (HPLC). Several experimental parameters such as kinds of UiO-66/PAN nanofibers, the amount of UiO-66/PAN nanofibers, the effect of solution pH, ionic strength and desorption conditions were intensively investigated. Under the optimal conditions, the linear ranges of the phytohormones were in the range of 0.06–60 ng/mL with correlation coefficients above 0.992. The limits of detection were between 0.01 ng/mL to 0.02 ng/mL. The interday and intraday precision (RSD) for three replicate extractions of the four phytohormones (15 ng/mL for each) was in the range of 1.5–5.6%. The established method was successfully applied for the determination of phytohormones in watermelon and mung bean sprouts samples. The results showed that the electrostatic interaction between the positively charged UiO-66 and anionic forms of phytohormones played an important role in the extraction of the phytohormones.

在这项工作中，首次将掺有金属-有机骨架颗粒的纤维（UiO-66 / PAN纳米纤维）用作移液器吸头固相萃取（PT-SPE）中的吸附剂。UiO-66 / PAN纳米纤维是通过简便的静电纺丝方法制备的，并通过傅里叶变换红外光谱，扫描电子显微镜，X射线衍射和氮吸附-脱附实验进行了表征。UiO-66 / PAN纳米纤维用于组装新型PT-SPE柱，用于测定四种植物激素，然后进行高效液相色谱（HPLC）。深入研究了诸如UiO-66 / PAN纳米纤维的种类，UiO-66 / PAN纳米纤维的数量，溶液pH值，离子强度和解吸条件的影响等实验参数。在最佳条件下 植物激素的线性范围为0.06–60 ng / mL，相关系数大于0.992。检测限在0.01 ng / mL至0.02 ng / mL之间。三种植物激素的三份重复提取（每份15 ng / mL）的日间和日内精度（RSD）在1.5-5.6％的范围内。所建立的方法已成功用于西瓜和绿豆芽样品中植物激素的测定。结果表明，带正电荷的UiO-66与阴离子形式的植物激素之间的静电相互作用在植物激素的提取中起重要作用。三种植物激素的三份重复提取（每份15 ng / mL）的日间和日内精度（RSD）在1.5-5.6％的范围内。所建立的方法已成功用于西瓜和绿豆芽样品中植物激素的测定。结果表明，带正电荷的UiO-66与阴离子形式的植物激素之间的静电相互作用在植物激素的提取中起重要作用。三种植物激素的三份重复提取（每份15 ng / mL）的日间和日内精度（RSD）在1.5-5.6％的范围内。所建立的方法已成功用于西瓜和绿豆芽样品中植物激素的测定。结果表明，带正电荷的UiO-66与阴离子形式的植物激素之间的静电相互作用在植物激素的提取中起重要作用。