The cytosine embedded copper based metal-organic framework (Bio-MOF) was synthesized by facile one-step sonochemical method by simply mixing of 4-4, biphenyldicarboxylic, cytosine and copper nitrate (Bio-Cu-H₂bpdc-Cy). The prepared bio-MOF was characterized by XRD, FTIR and FE-SEM techniques. The effect of Cu-H₂bpdc-Cy on the expression of the rsbA gene was evaluated in the clinical and standard proteus mirabilis and study of MIC of Cu-H₂bpdc-Cy by microdilution against them that have the rsbA gene. According to different concentrations of MIC, MBC concentrations was cultured on blood agar culture medium. Regarding to the concentration of MIC, gene expression changes were obtained by real-time PCR. MIC for standard and clinical strains of proteus mirabilis was 1.6 and 1.8 mg/ml, and also MBC was obtained to be 1.8 and 2.0 mg/ml, respectively. Finally, in the real time PCR method, expression of the rsbA gene in presences of bio-Cu-H₂bpdc-Cy was reduced, but has no effect on the gene expression of the Housekeeping DNA Gyrase-B gene. Considering the effect of Cu-H₂bpdc-Cy on the rsbA gene in proteus mirabilis bacteria, it is possible to use of Cu-H₂bpdc-Cy agent as a therapeutic supplement against this bacterium.

胞嘧啶嵌入的铜基金属有机骨架（Bio-MOF）是通过简单的一步式声化学方法，将4-4，联苯二甲酸，胞嘧啶和硝酸铜（Bio-Cu-H ₂ bpdc-Cy）简单混合而合成的。通过XRD，FTIR和FE-SEM技术对制备的生物MOF进行了表征。的Cu-H的效果₂ BPDC-CY上的表达rsbA基因在临床和标准进行评价奇异变形杆菌和MIC的研究的Cu-H ₂通过对具有rsbA基因的微稀释来对bpdc-Cy进行检测。根据MIC的不同浓度，在血琼脂培养基上培养MBC浓度。关于MIC的浓度，通过实时PCR获得基因表达变化。标准和临床变形杆菌的MIC分别为1.6和1.8 mg / ml，MBC分别为1.8和2.0 mg / ml。最后，在实时PCR方法中，在生物-Cu- H ₂ bpdc-Cy的存在下rsbA基因的表达降低了，但是对管家DNA促旋酶-B基因的基因表达没有影响。考虑Cu-H ₂ bpdc-Cy对奇异变形杆菌rsbA基因的影响细菌，有可能使用Cu-H ₂ bpdc-Cy试剂作为针对这种细菌的治疗性补充剂。