Phytosterols are bioactive components capable of reducing cholesterol level in serum and reducing risk of arteriosclerosis. In this study, conditions for the synthesis of maximum yield of phytosterol linoleic ester (PLE) was optimized and the physicochemical properties and in vitro bioaccessibility of the PLE were assessed. Under the optimized condition of 1:1.1 mol ratio of phytosterol and linoleoyl chloride at 80 °C for 1.5 h, the conversion rate of phytosterol reached 96.1%. Its solubility in oil increased 20 times, up to 33.8%. Also, peroxide value of PLE was much lower than linoleic acid (32.9 and 47.0 mmol/kg), which means better oxidative stability. Bioaccessibility of PLE was affected by time, concentration of bile extract, and dissolved medium. It was 4.93% alone, increased by 2.5 times compare to phytosterol; or 53.46% in oil, under the condition of 40 mg/mL bile extract for 120 min. In conclusion, under the tested condition, phytosterol conversion rate, its solubility in oil and bioaccessibility were improved significantly. The method showed great potential in manufacture high quality and quantity of PLE.

植物固醇是能够降低血清胆固醇水平并降低动脉硬化风险的生物活性成分。本研究优化了植物甾醇亚油酸酯（PLE）的最大合成条件，并对其理化性质和体外特性进行了研究。评估了PLE的生物可及性。在80℃下植物甾醇与亚油酰氯的摩尔比为1：1.1的最佳条件下1.5 h，植物甾醇的转化率达到96.1％。它在油中的溶解度增加了20倍，最高达到33.8％。而且，PLE的过氧化物值远低于亚油酸（32.9和47.0 mmol / kg），这意味着更好的氧化稳定性。PLE的生物可及性受时间，胆汁提取物浓度和溶解介质的影响。单独为4.93％，是植物甾醇的2.5倍；在40 mg / mL胆汁提取液中120分钟的条件下，在油中的含量为53.46％。综上所述，在试验条件下，植物甾醇转化率，其在油中的溶解度和生物利用度均得到显着提高。该方法在制造高质量和大量的PLE方面显示出巨大的潜力。