Active packaging is designed to interact with foods by releasing active components with biological properties, allowing the extension of foods shelf-life, being aromatic plants a source of active compounds. The main goal of this research was to develop a biodegradable whey protein based film, incorporated with an aromatic plant extract. Ethanolic extracts from rosemary and thyme were obtained from three commercial brands. The DPPH• scavenging activity assay showed that the rosemary extracts presented the highest antioxidant capacity. Thyme acetone extract had the highest antioxidant activity in the β-carotene bleaching assay. Three different mixtures of rosemary and thyme extracts with the highest antioxidant capacity showed a lower antioxidant capacity than the single extracts. The content of carnosic acid, carnosol and rosmarinic acid was determined in the three rosemary extracts, having the extract from the brand C shown the highest phenolics content. The extract showed antimicrobial activity against Staphylococcus aureus, Listeria monocytogenes and Clostridium perfringens. For the active film, 1% of the rosemary extract was incorporated into the whey protein film. The film presented antimicrobial activity against L. monocytogenes and S. aureus.

活性包装旨在通过释放具有生物学特性的活性成分与食品相互作用，从而延长食品的保质期，而芳香植物是活性化合物的来源。这项研究的主要目的是开发一种可生物降解的乳清蛋白基薄膜，并与一种芳香植物提取物结合在一起。迷迭香和百里香的乙醇提取物来自三个商业品牌。DPPH•清除活性试验表明，迷迭香提取物具有最高的抗氧化能力。百里香丙酮提取物在β-胡萝卜素漂白试验中具有最高的抗氧化活性。具有最高抗氧化能力的迷迭香和百里香提取物的三种不同混合物显示出比单一提取物更低的抗氧化能力。肌酸的含量 在三种迷迭香提取物中测定了鼠尾草酚和迷迭香酸的含量，其中品​​牌C的提取物显示出最高的酚含量。提取物显示出抗微生物活性金黄色葡萄球菌，单核细胞增生性李斯特菌和产气荚膜梭菌。对于活性膜，将1％的迷迭香提取物掺入乳清蛋白膜中。该膜对单核细胞增生李斯特菌和金黄色葡萄球菌具有抗菌活性。