A fast responsive and two photon fluorescent probe (HCyNAc) for carboxylesterase (CaE) has been designed based on the D–π–A structure of hemicyanine and naphthalene derivatives. After enzymatic reaction, HCyNAc enabled light-up fluorescence assay of CaE over other biologically-relevant species and enzymes, including ROS. Two-photon imaging of endogenous CaE was confirmed in HeLa cells using HCyNAc under 800 nm NIR excitation.

中文翻译：

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快速反应，高选择性和发光的荧光探针，用于活细胞中羧酸酯酶的双光子成像†

基于半菁和萘衍生物的D–π–A结构，设计了一种用于羧酸酯酶（CaE）的快速响应和两个光子荧光探针（HCyNAc）。酶促反应后，HCyNAc可以对CaE进行其他生物学相关的物种和酶（包括ROS）的亮光荧光测定。在800 nm NIR激发下，使用HCyNAc在HeLa细胞中证实了内源CaE的双光子成像。