Biomimetic membranes create opportunities for various applications, including the possibility of replacing interacting cells in a cell–cell contact. Here we have fractionated synthetic membranes using metal nano-grid structures where EphrinA5 (EA5), a neuronal adhesion promoter, was anchored via its Fc domain (immunoglobulin G (IgG)-domain). FRAP experiments were performed to check the confinement of the synthetic membrane within these nano-structures. Rat cortical primary neurons were cultured and live cell imaging techniques were used to monitor the neuronal interaction with these fractionated synthetic membranes. Computational imaging analysis of the corresponding images elucidated interesting details of the cellular behavior. The phenotypic cellular response on these nano-membrane fractions was found to be similar to that on non-fractionated synthetic membranes indicating that although the number of focal adhesion points was low (due to the reduced EA5 number) in the nano-sized membrane patches perhaps some other factors like metal grid boundaries might be playing a role in rendering the similarity.

中文翻译：

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纳米图案EA5-POPC合成膜上神经元的粘附和生长†

仿生膜为各种应用创造了机会，包括在细胞间接触中替换相互作用细胞的可能性。在这里，我们使用金属纳米网格结构分离了合成膜，其中神经元粘附促进剂EphrinA5（EA5）通过其Fc结构域（免疫球蛋白G（IgG）结构域）。进行了FRAP实验，以检查合成膜在这些纳米结构内的限制。培养大鼠皮质原代神经元，并使用活细胞成像技术监测与这些分离的合成膜的神经元相互作用。相应图像的计算成像分析阐明了细胞行为的有趣细节。发现在这些纳米膜级分上的表型细胞反应与在未分离的合成膜上的表型细胞反应相似，这表明尽管纳米级膜片中的粘着点数很低（由于减少了EA5值）。其他一些因素（例如金属网格边界）可能在呈现相似性方面发挥了作用。