SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range,Chemical Communications

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SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range
Chemical Communications ( IF 4.3 ) Pub Date : 2018-02-20 00:00:00 , DOI: 10.1039/c7cc08740c
Y. G. Ermakova _{1,

2,

2,

3,

4} , V. V. Pak _{1,

2,

3} , Y. A. Bogdanova _{1,

2,

3} , A. A. Kotlobay _{1,

2,

3} , I. V. Yampolsky _{1,

2,

3} , A. G. Shokhina _{1,

2,

3} , A. S. Panova _{1,

2,

3,

5,

6} , R. A. Marygin _{1,

2,

3} , D. B. Staroverov _{1,

2,

3} , D. S. Bilan _{1,

2,

2,

3,

4} , H. Sies _{7,

8,

9,

10,

11} , V. V. Belousov _{1,

2,

2,

3,

4}

Affiliation

We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pK_a, which responds to pH changes in different cellular compartments. SypHer3s was successfully utilized for imaging the pH dynamics in mitochondria of living neurons and in quantitative pH measurement in zebrafish embryos.

中文翻译：

SypHer3s：一种遗传编码的荧光比率式探针，具有增强的亮度和改进的动态范围

我们设计了一种遗传编码的比例荧光探针SypHer3s，该探针具有增强的亮度和优化的p K _a，可响应不同细胞区室中的pH变化。SypHer3s已成功用于成像活神经元线粒体中的pH动态以及在斑马鱼胚胎中进行定量pH测量。

更新日期：2018-03-15

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