Eight lactic acid bacterial (LAB) strains were newly isolated from fresh shrimps (Penaeus vannamei and Palaemon serratus) and characterised by phenotypic and molecular tests. These isolates, identified as Enterococcus lactis producing the enterocins A, B and/or P, displayed interesting antagonistic activities against pathogenic bacteria and fungi. Regarding biotechnological properties, E. lactis strains had generally great redox potentials, good proteolytic activities (P < 0.05) and medium acidification rates. Evaluated by the API ZYM system, two strains named Q1 and 4CP3 exhibited good enzymatic activities: leucine, valine and cystine arymalidases, Naphthol-AS-BI-phosphohydrolase and β-galactosidase. For probiotic assessment, the examined strains were found to resist low pH and bile salts with presence of gastric and digestive enzymes. Likewise, they indicated potentialities of adhesion and colonisation in the intestines. All strains were sensitive to clinically important antibiotics and no PCR signals were detected for any of the common antibiotic resistance genes such as vanA and vanB for vancomycin. Furthermore, virulence traits including the presence of virulence factors, haemolysin and gelatinase, were not detected. This work suggests that our E. lactis strains can be considered as promising candidates for future applications as a starter, adjunct or protective cultures or could constitute new potential probiotics.

八乳酸细菌（LAB）菌株新从新鲜虾（隔绝南美白对虾和长臂锯）并且其特征在于表型和分子测试。这些分离物被鉴定为产生肠球蛋白A，B和/或P的乳酸肠球菌，对病原菌和真菌表现出令人感兴趣的拮抗活性。关于生物技术特性，乳酸大肠杆菌通常具有很大的氧化还原电位，良好的蛋白水解活性（P <0.05）和中等酸化率。由API ZYM系统评估，两个名为Q1和4CP3的菌株均表现出良好的酶促活性：亮氨酸，缬氨酸和胱氨酸偶氮菌，萘酚-AS-BI-磷酸水解酶和β-半乳糖苷酶。为了进行益生菌评估，发现所检查的菌株在存在胃和消化酶的情况下能抵抗低pH值和胆汁盐。同样，他们指出了肠道内粘附和定植的潜力。所有菌株均对临床上重要的抗生素敏感，并且未检测到任何常见抗生素抗性基因（例如万古霉素的vanA和vanB）的PCR信号。此外，未检测到毒力特征，包括毒力因子，溶血素和明胶酶的存在。这项工作表明我们乳酸杆菌菌株可以被视为有前途的，辅助的或保护性的培养物，有可能成为未来应用的候选物，或者可以构成新的潜在益生菌。