New generation inert ionic liquid (iIL) GC columns IL60i, IL76i and IL111i, comprising phosphonium or imidazolium cationic species, were investigated for separation of fatty acid methyl esters (FAME). In general, the iIL phases provide comparable retention times to their corresponding conventional columns, with only minor selectivity differences. The average tailing factors and peak widths were noticeably improved (reduced) for IL60i and IL76i, while they were slightly improved for IL111i. Inert IL phase columns were coupled with conventional IL columns in comprehensive two-dimensional GC (GC × GC) with a solid-state modulator which offers variable modulation temperature (T_M), programmable T_M during analysis and trapping stationary phase material during the trap/release (modulation) process, independent of oven T and column sets. Although IL phases are classified as polar, relative polarity of the two phases comprising individual GC × GC column sets permits combination of less-polar IL/polar IL and polar IL/less-polar IL column sets; it was observed that a polar/less-polar column set provided better separation of FAME. A higher first dimension (¹D) phase polarity combined with a lower ²D phase polarity, for instance ¹D IL111i with ²D IL59 gave the best result; the greater difference in ¹D/²D phase polarity results in increasing occupancy of peak area in the 2D space. The IL111i/IL59 column set was selected for analysis of fatty acids in fat and oil products (butter, margarine, fish oil and canola oil). Compared with the conventional IL111, IL111i showed reduced column bleed which makes this more suited to GC × GC analysis of FAME. The proposed method offers a fast profiling approach with good repeatability of analysis of FAME.

为了分离脂肪酸甲酯（FAME），对包含phospho或咪唑鎓阳离子物质的新一代惰性离子液体（iIL）GC色谱柱IL60i，IL76i和IL111i进行了研究。通常，iIL相可提供与其相应的常规色谱柱相当的保留时间，且选择性差异很小。IL60i和IL76i的平均拖尾因子和峰宽明显改善（降低），而IL111i的平均拖尾因子和峰宽略有改善。惰性IL相色谱柱与常规IL色谱柱在具有固态调制器的全面二维GC（GC×GC）中耦合，该固态调制器提供可变的调制温度（T _M），可编程的T _M在分析和捕获/释放（调制）过程中捕获固定相材料时，与柱温箱和色谱柱组无关。尽管IL相被分类为极性相，但包括单个GC×GC色谱柱组的两相的相对极性允许将极性较小的IL /极性IL和极性IL /极性较小的IL色谱柱组合使用。据观察，极性/非极性色谱柱组可提供更好的FAME分离效果。较高的第一维（¹ D）相极性与较低的² D相极性相结合，例如¹ D IL111i和² D IL59可产生最佳结果。¹ D / ^2的差异越大D相极性导致2D空间中峰面积的增加。选择IL111i / IL59色谱柱组用于分析油脂产品（黄油，人造黄油，鱼油和低芥酸菜子油）中的脂肪酸。与传统的IL111相比，IL111i减少了柱流失，这使其更适合于FAME的GC×GC分析。所提出的方法提供了一种快速分析方法，具有良好的FAME分析可重复性。