Tracking the Dynamic Folding and Unfolding of RNA G‐Quadruplexes in Live Cells,Angewandte Chemie International Edition

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Tracking the Dynamic Folding and Unfolding of RNA G‐Quadruplexes in Live Cells
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2018-03-13 , DOI: 10.1002/anie.201801999
Xiu-Cai Chen ₁ , Shuo-Bin Chen ₁ , Jing Dai ₁ , Jia-Hao Yuan ₁ , Tian-Miao Ou ₁ , Zhi-Shu Huang ₁ , Jia-Heng Tan ₁

Affiliation

Because of the absence of methods for tracking RNA G‐quadruplex dynamics, especially the folding and unfolding of this attractive structure in live cells, understanding of the biological roles of RNA G‐quadruplexes is so far limited. Herein, we report a new red‐emitting fluorescent probe, QUMA‐1, for the selective, continuous, and real‐time visualization of RNA G‐quadruplexes in live cells. The applications of QUMA‐1 in several previously intractable applications, including live‐cell imaging of the dynamic folding, unfolding, and movement of RNA G‐quadruplexes and the visualization of the unwinding of RNA G‐quadruplexes by RNA helicase have been demonstrated. Notably, our real‐time results revealed the complexity of the dynamics of RNA G‐quadruplexes in live cells. We anticipate that the further application of QUMA‐1 in combination with appropriate biological and imaging methods to explore the dynamics of RNA G‐quadruplexes will uncover more information about the biological roles of RNA G‐quadruplexes.

中文翻译：

跟踪活细胞中RNA G四联体的动态折叠和展开

由于缺乏跟踪RNA G-四链体动力学的方法，尤其是这种有吸引力的结构在活细胞中的折叠和展开，因此迄今为止对RNA G-四链体的生物学作用的了解还很有限。本文中，我们报告了一种新的红色荧光探针QUMA-1，用于选择性，连续和实时可视化活细胞中的RNA G四链体。QUMA-1的应用在一些以前难以解决的应用中，已经证明了RNA G四链体的动态折叠，展开和运动的活细胞成像，以及通过RNA解旋酶可视化RNA G四链体的展开。值得注意的是，我们的实时结果揭示了活细胞中RNA G四联体动力学的复杂性。我们预计，将QUMA-1与适当的生物学和成像方法结合使用以探索RNA G-四链体动力学的进一步应用将揭示出有关RNA G-四链体的生物学作用的更多信息。

更新日期：2018-03-13

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