Highly Elastic Biodegradable Single-Network Hydrogel for Cell Printing.,ACS Applied Materials & Interfaces

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Highly Elastic Biodegradable Single-Network Hydrogel for Cell Printing.
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2018-03-17 , DOI: 10.1021/acsami.8b01294
Cancan Xu _{1,

2} , Wenhan Lee ₃ , Guohao Dai ₃ , Yi Hong _{1,

2}

Affiliation

Cell printing is becoming a common technique to fabricate cellularized printed scaffold for biomedical application. There are still significant challenges in soft tissue bioprinting using hydrogels, which requires live cells inside the hydrogels. Moreover, the resilient mechanical properties from hydrogels are also required to mechanically mimic the native soft tissues. Herein, we developed a visible-light cross-linked, single-network, biodegradable hydrogel with high elasticity and flexibility for cell printing, which is different from previous highly elastic hydrogel with double-network and two components. The single-network hydrogel using only one stimulus (visible light) to trigger gelation can greatly simplify the cell printing process. The obtained hydrogels possessed high elasticity, and their mechanical properties can be tuned to match various native soft tissues. The hydrogels had good cell compatibility to support fibroblast growth in vitro. Various human cells were bioprinted with the hydrogels to form cell-gel constructs, in which the cells exhibited high viability after 7 days of culture. Complex patterns were printed by the hydrogels, suggesting the hydrogel feasibility for cell printing. We believe that this highly elastic, single-network hydrogel can be simply printed with different cell types, and it may provide a new material platform and a new way of thinking for hydrogel-based bioprinting research.

中文翻译：

用于细胞打印的高弹性可生物降解单网络水凝胶。

细胞打印正在成为制造生物医学应用细胞打印支架的常用技术。使用水凝胶进行软组织生物打印仍然面临重大挑战，这需要水凝胶内有活细胞。此外，还需要水凝胶的弹性机械性能来机械模拟天然软组织。在此，我们开发了一种可见光交联、单网络、可生物降解的水凝胶，具有高弹性和灵活性，用于细胞打印，这与之前的双网络和两种组分的高弹性水凝胶不同。仅使用一种刺激（可见光）来触发凝胶化的单网络水凝胶可以大大简化细胞打印过程。获得的水凝胶具有高弹性，并且可以调整其机械性能以匹配各种天然软组织。水凝胶具有良好的细胞相容性，可支持体外成纤维细胞的生长。使用水凝胶对各种人体细胞进行生物打印，形成细胞凝胶结构，其中细胞在培养 7 天后表现出高活力。水凝胶打印出复杂的图案，表明水凝胶用于细胞打印的可行性。我们相信这种高弹性、单网络水凝胶可以简单地打印不同类型的细胞，它可能为基于水凝胶的生物打印研究提供新的材料平台和新的思维方式。

更新日期：2018-02-16

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