High purity diacylglycerols (DAG) rich in medium-chain fatty acid diacylglycerols (MCD) and medium- and long-chain fatty acid diacylglycerols (MLCD) were prepared via the enzymatic esterification of monoacylglycerols (MAG) with caprylic acid followed by molecular distillation (MD), solvent fraction and low-temperature centrifugation. The content of DAG in the crude product was 44.8 ± 0.1%, under the selected esterification conditions, which were MAGs/caprylic acid mole ratio of 1:3, reaction temperature of 65 °C, reaction time of 30 min and enzyme load of 5 wt%. Subsequently, the one-step MD and solvent fraction in methanol/ethanol increased the DAG content to 61.3 ± 0.8%. Eventually, the product containing 86.6 ± 0.6% of DAG with 39.3 ± 1.3% of MCD and 47.3 ± 0.6% of MLCD was obtained by the methanol crystallization at 0 °C with a water content of 9 wt% and a 1:3 ratio of glycerides/methanol (v/v) followed by the centrifugation separation at 0 °C.

富含中链脂肪酸二酰基甘油（MCD）和中链和长链脂肪酸二酰基甘油（MLCD）的高纯度二酰基甘油（DAG）是通过单酰基甘油（MAG）与辛酸的酶促酯化反应，然后进行分子蒸馏（MD）制备的），溶剂馏分和低温离心。在选择的酯化条件下，MAGs /辛酸摩尔比为1：3，反应温度为65°C，反应时间为30分钟，酶负荷为5，在粗产物中DAG的含量为44.8±0.1％。重量％。随后，一步法MD和溶剂在甲醇/乙醇中的馏分将DAG含量提高到61.3±0.8％。最终，该产品含有86.6±0.6％的DAG，39.3±1.3％的MCD和47.3±0。