Lateral flow assay with pressure meter readout for rapid point-of-care detection of disease-associated protein†,Lab on a Chip

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Lateral flow assay with pressure meter readout for rapid point-of-care detection of disease-associated protein†
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-02-16 00:00:00 , DOI: 10.1039/c8lc00010g
Bingqian Lin _{1,

2,

3,

4,

5} , Zhichao Guan _{6,

7,

8} , Yanling Song _{8,

9,

10,

11,

12} , Eunyeong Song _{1,

2,

3,

4,

5} , Zifei Lu _{1,

2,

3,

4,

5} , Dan Liu _{1,

2,

3,

4,

5} , Yuan An _{1,

2,

3,

4,

5} , Zhi Zhu _{1,

2,

3,

4,

5} , Leiji Zhou _{1,

2,

3,

4,

5} , Chaoyong Yang _{1,

2,

3,

4,

5}

Affiliation

Paper-based assays such as lateral flow assays are good candidates for portable diagnostics owing to their user-friendly format and low cost. In terms of analytical detection, lateral flow assays usually require dedicated instruments to obtain quantitative results. Here we demonstrate a lateral flow assay with handheld pressure meter readout for the rapid detection of disease-related protein with high sensitivity and selectivity. Based on the pressure change produced by the catalytic reaction of Pt nanoparticles related to the concentration of the target, a quantitative reaction platform was established. During the lateral flow assay, the Pt nanoparticles are aggregated in the test line to form a gray band by biomolecular recognition and finally convert the recognition signal into highly sensitive pressure readout for quantitative analysis. Without sophisticated instrumentation and complicated operations, the whole detection process can be completed within 20 minutes. The limit of detection for myoglobin (2.9 ng mL⁻¹ in diluted serum samples) meets the requirements of clinical monitoring. With the advantages of low cost, ease of operation, high sensitivity and selectivity, the method represents a versatile platform for point-of-care testing of disease biomarkers.

中文翻译：

带有压力表读数的侧向流动测定法可用于即时检测疾病相关蛋白†

纸质化验（例如侧向流动化验）由于其用户友好的格式和低成本而成为便携式诊断的理想选择。在分析检测方面，侧流分析通常需要专用仪器才能获得定量结果。在这里，我们展示了具有手持式压力计读数的侧向流动检测技术，可以高灵敏度和高选择性快速检测疾病相关蛋白。基于Pt纳米颗粒催化反应产生的与目标浓度有关的压力变化，建立了定量反应平台。在侧向流动测定中，Pt纳米颗粒通过生物分子识别在测试线中聚集，形成一条灰色带，最后将识别信号转换为高度敏感的压力读数，以进行定量分析。无需复杂的仪器和复杂的操作，整个检测过程可以在20分钟内完成。肌红蛋白的检出限（2.9 ng mL稀释的血清样品中的^-1）符合临床监测的要求。该方法具有成本低，易于操作，灵敏度高和选择性高的优点，代表了一种用于疾病生物标志物即时检验的多功能平台。

更新日期：2018-02-16

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