Rapid, Stoichiometric, Site-Specific Modification of Aldehyde-Containing Proteins Using a Tandem Knoevenagel-Intra Michael Addition Reaction,Bioconjugate Chemistry

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Rapid, Stoichiometric, Site-Specific Modification of Aldehyde-Containing Proteins Using a Tandem Knoevenagel-Intra Michael Addition Reaction
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2018-02-15 00:00:00 , DOI: 10.1021/acs.bioconjchem.8b00086
Jian Yu ₁ , Da Shen ₁ , Hanjie Zhang ₁ , Zheng Yin ₁

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A site-specific modification of aldehyde-containing proteins using a tandem Knoevenagel-intra Michael addition reaction was developed. The reaction featured fast kinetics (50 M^–1 s^–1) and favorable stoichiometry. Various functionalities could be introduced into the protein with little impact on its function and conformation. The reaction was successfully applied in the labeling of living cells.

中文翻译：

使用串联Knoevenagel-Michael内部加成反应对含醛蛋白进行快速，化学计量，位点特异性修饰

开发了使用串联Knoevenagel-Michael内部加成反应的含醛蛋白质的位点特异性修饰。该反应具有快速的动力学（50 M ^–1 s ^–1）和良好的化学计量比。可以将各种功能引入蛋白质中，而对其功能和构象影响很小。该反应已成功地应用于活细胞的标记。

更新日期：2018-02-15

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