Candida utilis yeast, a rich source of proteins and vitamin B-complex was cultivated on cassava peel, a food processing waste, which was first liquefied with Termamyl 120 L α-amylase enzyme. The cultivation process was optimized simultaneously with saccharification with Novo AMG 300 L amyloglucosidase using response surface methodology approach. The design involved three duration of enzyme hydrolysis (0, 4.5 and 9 h) prior to inoculation with Candida utilis representing varied degrees of hydrolysis (0, 50 and 100%) and initial pH (4.5, 5.0 and 5.5). Measured responses were change in yeast protein ranging from 1.13 to 1.91 mg/mL, change in cell concentration ranging from 2.30 to 3.90 mg/mL and specific growth rate ranging from 0.21–0.51.100% hydrolysis and initial pH of 5.0 gave the highest changes in yeast protein (1.92 mg/mL) and cell concentration (3.90 mg/mL); 100% hydrolysis and pH 5.5 gave the highest specific growth rate. The optimal solution was obtained at pH of 5.5 and 100% degree of hydrolysis with a degree of desirability of 0.8. The cultivation of Candida utilis yeast on cassava peel is of high significance to food and agro-based industries for the production of value added products, waste disposal and valorisation.

Industrial relevance

Cassava peel is a major waste product from cassava processing industry which is faced with an enormous challenge regarding its disposal. This study revealed that Candida utilis can be cultivated successfully on cassava peel slurry; the cultivation of this yeast on cassava peel is of high significance to food and agro-based industries for the production of value added products waste disposal and valorisation.

中文翻译：

---

木薯皮同时发酵糖化util假丝酵母

木薯果皮（一种食品加工废料）上培养了丰富的蛋白质假丝酵母和复合维生素B复合物，首先将其用Termamyl 120 Lα-淀粉酶液化。使用响应面方法将糖化过程与Novo AMG 300 L淀粉葡糖苷酶同时糖化进行优化。设计涉及在用假丝酵母接种之前的三个酶水解时间（0、4.5和9小时）代表不同程度的水解（0、50和100％）和初始pH（4.5、5.0和5.5）。测得的响应是​​酵母蛋白质的变化范围为1.13至1.91 mg / mL，细胞浓度的变化范围为2.30至3.90 mg / mL，比生长速率范围为0.21-0.51.100％水解，初始pH为5.0时变化最大酵母蛋白（1.92 mg / mL）和细胞浓度（3.90 mg / mL）；100％水解和pH 5.5给出了最高的比生长速率。在pH为5.5且水解度为100％且期望度为0.8的情况下获得了最佳溶液。木薯果皮上的假丝酵母菌的培养对于食品和农业产业的增值产品生产，废物处理和增值具有重要意义。

行业相关性

木薯果皮是木薯加工业的主要废品，其处置面临着巨大挑战。这项研究表明，在木薯果皮浆液中可以成功地培养出假丝酵母。木薯果皮上这种酵母的培养对食品和农业工业产生增值产品废物处理和增值具有重要意义。