A Versatile and Robust Approach to Stimuli-Responsive Protein Multilayers with Biologically Enabled Unique Functions,Biomacromolecules

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A Versatile and Robust Approach to Stimuli-Responsive Protein Multilayers with Biologically Enabled Unique Functions
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-02-14 00:00:00 , DOI: 10.1021/acs.biomac.8b00190
Xue-Jian Zhang _{1,

2} , Xiao-Wei Wang ₁ , Xiao-Di Da ₁ , Yanlin Shi ₃ , Chunli Liu ₃ , Fei Sun ₄ , Shuguang Yang ₂ , Wen-Bin Zhang ₁

Affiliation

Protein-based materials call for innovative processing techniques to integrate their unique biologically enabled functions with other materials of complementary features. Herein, we report the covalent protein layer-by-layer assembly via orthogonal “Tag-Catcher” reactions as a facile and robust approach to make entirely protein-based multilayers on a variety of substrates. Programmed assembly of native telechelic proteins not only endows the materials valuable stimuli-sensitive behaviors, but also unique properties unparalleled by any synthetic counterparts. As proof of concept, super uranyl-binding protein (SUP) is immobilized on silica gel by this method with tunable capacity and enhanced capability for uranyl sequestration. Not only is the capturing performance enhanced in the multilayer setup, it also confers resilience to recycling, allowing efficient harvest of uranyl with an average of ∼90% and ∼60% recovery rate in over 10 cycles from water and synthetic seawater, respectively. The approach is the first entirely protein-based multilayers covalently assembled by the layer-by-layer method. It provides a platform for immobilizing proteins with synergistic enhancement of function and resilience and expands the scope and capability of genetically encoded protein-based materials.

中文翻译：

具有生物启用的独特功能的刺激响应蛋白多层的多功能和鲁棒方法。

基于蛋白质的材料需要创新的加工技术，以将其独特的具有生物功能的功能与其他具有互补功能的材料整合在一起。在本文中，我们报告了通过正交的“ Tag-Catcher”反应进行共价蛋白逐层组装的方法，该方法是在各种底物上完全基于蛋白质的多层结构的便捷而强大的方法。天然远螯蛋白的程序化组装不仅赋予材料有价值的刺激敏感性行为，而且赋予其独特的特性，这是任何合成类似物所无法比拟的。作为概念的证明，通过该方法，可调节的能力和增强的铀酰螯合能力将超铀酰结合蛋白（SUP）固定在硅胶上。多层设置不仅提高了捕获性能，还赋予了回收利用弹性，可以在十个以上的循环中分别从水和合成海水中高效收获铀酰，回收率分别约为90％和60％。该方法是通过逐层方法共价组装的第一个完全基于蛋白质的多层。它提供了一个平台，可以协同增强功能和复原力来固定蛋白质，并扩展了遗传编码的基于蛋白质的材料的范围和能力。

更新日期：2018-02-14

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