Detection of Adrenaline in Blood Plasma as Biomarker for Adrenal Venous Sampling,Electroanalysis

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Detection of Adrenaline in Blood Plasma as Biomarker for Adrenal Venous Sampling
Electroanalysis ( IF 2.7 ) Pub Date : 2018-02-14 , DOI: 10.1002/elan.201800026
Denise Molinnus _{1,

2} , Gabriel Hardt ₁ , Petra Siegert ₁ , Holger S. Willenberg ₃ , Arshak Poghossian _{1,

4} , Michael Keusgen ₂ , Michael J. Schöning _{1,

4}

Affiliation

An amperometric bi‐enzyme biosensor based on substrate recycling principle for the amplification of the sensor signal has been developed for the detection of adrenaline in blood. Adrenaline can be used as biomarker verifying successful adrenal venous sampling procedure. The adrenaline biosensor has been realized via modification of a galvanic oxygen sensor with a bi‐enzyme membrane combining a genetically modified laccase and a pyrroloquinoline quinone‐dependent glucose dehydrogenase. The measurement conditions such as pH value and temperature were optimized to enhance the sensor performance. A high sensitivity and a low detection limit of about 0.5–1 nM adrenaline have been achieved in phosphate buffer at pH 7.4, relevant for measurements in blood samples. The sensitivity of the biosensor to other catecholamines such as noradrenaline, dopamine and dobutamine has been studied. Finally, the sensor has been successfully applied for the detection of adrenaline in human blood plasma.

中文翻译：

血浆中肾上腺素的检测作为肾上腺静脉取样的生物标志物

已开发出一种基于底物循环原理的安培双酶生物传感器，用于放大传感器信号，用于检测血液中的肾上腺素。肾上腺素可以用作生物标志物，以验证肾上腺静脉采样程序是否成功。肾上腺素生物传感器是通过用结合了基因修饰的漆酶和吡咯并喹啉醌依赖性葡萄糖脱氢酶的双酶膜修饰电流氧传感器而实现的。优化了pH值和温度等测量条件，以增强传感器性能。在pH 7.4的磷酸盐缓冲液中，已经获得了高灵敏度和低检测限（约0.5–1 nM肾上腺素），这与血样的测量有关。生物传感器对其他儿茶酚胺（例如去甲肾上腺素，已经研究了多巴胺和多巴酚丁胺。最终，该传感器已成功应用于人体血浆中肾上腺素的检测。

更新日期：2018-02-14

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