The wide application of titanium dioxide nanoparticles and phosphorus in the manufacturing of many industrial products mainly used in agricultural sector has resulted in the release of considerable amounts of these compounds into freshwater aquatic ecosystem. These compounds may cause some unexpected effects to aquatic organisms. This study assessed the binary effects of Titanium nanoparticles (nTiO₂) and Phosphorus on Chlorella ellipsoides. Toxicological assay test of the compounds nTiO₂ (1.25 μM) alone and the combination of Titanium dioxide (1.25 μM) and Phosphorus (16, 32, 80, 160, 240 μM) was assessed, after 96 h exposures, for optical density (OD₆₈₀), specific growth rate, chlorophyll levels and lipid peroxidation via Malondialdehyde (MDA) activity. Superoxide dismutase (SOD), peroxidase (POD) and glutathione-s-transferase (GST) activities were also measured. Two-way ANOVA showed a significant interaction (P < 0.05) between binary mixture. Co-exposure showed a decreased phosphorus bioconcentration in the microalgae with significant increase (P < 0.05) in chlorophyll a/b and total chlorophyll contents. A significant decrease (P < 0.05) in specific growth rate and optical density were recorded whereas, antioxidant enzymes (MDA, SOD, POD, GST) activities were significantly (P < 0.05) increased. These results showed that the addition of nTiO₂ to Phosphorus affected the physiology of microalgae and should be of great concern for freshwater biodiversity.

二氧化钛纳米颗粒和磷在许多主要用于农业领域的工业产品生产中的广泛应用已导致大量此类化合物释放到淡水水生生态系统中。这些化合物可能对水生生物造成某些意想不到的影响。这项研究评估了钛纳米粒子（nTiO ₂）和磷对小球藻的二元效应。暴露96 h后，评估了单独的化合物nTiO ₂（1.25μM ）和二氧化钛（1.25μM ）和磷（16、32、80、160、240μM ）的组合的毒理学试验测试的光密度（OD ₆₈₀），比生长速率，叶绿素水平和通过丙二醛（MDA）活性引起的脂质过氧化作用。还测量了超氧化物歧化酶（SOD），过氧化物酶（POD）和谷胱甘肽S-转移酶（GST）的活性。二元方差分析表明二元混合物之间存在显着的相互作用（P <0.05）。共同暴露表明，微藻中的磷生物浓度降低，叶绿素a / b和总叶绿素含量显着增加（P <0.05）。记录到其比生长速率和光密度显着下降（P <0.05），而抗氧化酶（MDA，SOD，POD，GST）的活性显着增加（P <0.05）。这些结果表明，向磷中添加nTiO ₂会影响微藻的生理，应引起淡水生物多样性的极大关注。