pH-Independent two-photon fluorescent lysotrackers for real-time monitoring autophagy†,Journal of Materials Chemistry B

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pH-Independent two-photon fluorescent lysotrackers for real-time monitoring autophagy†
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2018-02-14 00:00:00 , DOI: 10.1039/c8tb00229k
Wenjuan Wang _{1,

2,

3,

4} , Peng Ning _{1,

2,

3,

4} , Qi Wang _{1,

2,

3,

4} , Wan Zhang _{1,

2,

3,

4} , Jun Jiang _{1,

2,

3,

4} , Yan Feng _{1,

2,

3,

4} , Xiangming Meng _{1,

2,

3,

4}

Affiliation

Two carbazole-based two-photon fluorescent lysotrackers with different electron-donating groups (a methoxyl group for Lyso-MCO and a dimethylamino group for Lyso-NCO, respectively) have been developed from simple starting materials via an only 2-step procedure. Both of them exhibit pH-independent and specific lysosome location with a rapid staining rate, high photostability and deep issue penetration along with large two-photon absorption action cross-sections. By virtue of the better two-photon absorption properties of Lyso-NCO, it was chosen to visually monitor lysosomal tracking and autophagy. Compared with the approach of GFP-LC3 for autophagy detection, lysotracker Lyso-NCO achieved efficient and real-time visualization of the membrane fusion period in the autophagy process through detecting the level of the co-localization coefficients between Lyso-NCO and Mitotracker Red FM (MTR) in live cells.

中文翻译：

独立于pH值的双光子荧光溶血纤溶酶，用于实时监测自噬†

两个基于咔唑的双光子具有不同的给电子基团（对于甲氧基基团的荧光lysotrackers溶血MCO和二甲基氨基溶血NCO分别）已经从简单的起始原料开发通过一个仅两步骤过程。它们都表现出不依赖于pH值和特定的溶酶体位置，具有快速的染色速率，高的光稳定性和深度的穿透力以及较大的双光子吸收作用截面。由于Lyso-NCO具有更好的双光子吸收特性，因此选择它在视觉上监测溶酶体的追踪和自噬。与GFP-LC3进行自噬检测的方法相比，溶血追踪器Lyso-NCO通过检测活细胞中Lyso-NCO和Mitotracker Red FM（MTR）之间的共定位系数水平，实现了自噬过程中膜融合期的高效，实时可视化。

更新日期：2018-02-14

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