In the present study, a novel model of type-I counter-current chromatography was developed to simplify the separation column manufacture and extend its application ranges. In this system, a multilayer coil was used as the separation column of type-I counter-current chromatography for the first time. The chromatographic performance of this apparatus was evaluated in terms of retention of the stationary phase (Sf), theoretical plate (N) and peak resolution (Rs) using dipeptides and DNP-amino acids as test samples with two classic solvent systems composed of 1-butanol-acetic acid-water (4:1:5, v/v) (BAW) and hexane-ethyl acetate-methanol-0.1 M HCl (1:1:1:1, v/v) (HEMW), respectively. The results indicated that the performance was optimal at the revolution speed of 200 rpm. The lower flow rate was more beneficial to retention of stationary phase and peak resolution. Over all results of our study revealed that the new type-I counter-current chromatography with a multilayer coil has a high application potential.

在本研究中，开发了一种新型的I型逆流色谱模型，以简化分离柱的制造并扩展其应用范围。在该系统中，多层线圈首次用作I型逆流色谱法的分离柱。使用二肽和DNP-氨基酸作为测试样品，并使用由1-组成的两种经典溶剂系统，根据固定相的保留（Sf），理论塔板数（N）和峰分离度（Rs）评估了该仪器的色谱性能。丁醇-乙酸-水（4：1：5，v / v）（BAW）和己烷-乙酸乙酯-甲醇-0.1 M HCl（1：1：1：1，v / v）（HEMW）。结果表明，在200 rpm的转速下性能最佳。较低的流速更有利于保留固定相和峰分离度。总体而言，我们的研究结果表明，带有多层线圈的新型I型逆流色谱具有很高的应用潜力。