In the present study, a novel model of type-I counter-current chromatography was developed to simplify the separation column manufacture and extend its application ranges. In this system, a multilayer coil was used as the separation column of type-I counter-current chromatography for the first time. The chromatographic performance of this apparatus was evaluated in terms of retention of the stationary phase (Sf), theoretical plate (N) and peak resolution (Rs) using dipeptides and DNP-amino acids as test samples with two classic solvent systems composed of 1-butanol-acetic acid-water (4:1:5, v/v) (BAW) and hexane-ethyl acetate-methanol-0.1 M HCl (1:1:1:1, v/v) (HEMW), respectively. The results indicated that the performance was optimal at the revolution speed of 200 rpm. The lower flow rate was more beneficial to retention of stationary phase and peak resolution. Over all results of our study revealed that the new type-I counter-current chromatography with a multilayer coil has a high application potential.

在本研究中，开发了一种新型I型逆流色谱模型，以简化分离柱的制造并扩展其应用范围。该系统首次采用多层线圈作为I型逆流色谱分离柱。使用二肽和 DNP-氨基酸作为测试样品，使用由 1-分别为丁醇-乙酸-水（4:1:5，v/v）（BAW）和己烷-乙酸乙酯-甲醇-0.1 M HCl（1:1:1:1，v/v）（HEMW）。结果表明，转速为200 rpm时性能最佳。较低的流速更有利于固定相的保留和峰分辨率。总的来说，我们的研究结果表明，具有多层线圈的新型 I 型逆流色谱具有很高的应用潜力。