The effect of 10 essential oil (EO) components on biofilm formation for vegetable spoilage Erwinia carotovora and Pseudomonas fluorescens at sub-MICs were investigated. Chromobacterium violaceum CV026 (CV026) was selected as quorum sensing (QS) biosensor bacterium. The swimming, swarming, twitching motility, exopolysaccharide (EPS) production and biofilm formation were determined. All tested EO components showed QS inhibition of CV026, E. carotovora and P. fluorescens. Biofilms were inhibited by all EO components by inhibiting their motilities and EPS production. Salicylic acid and thymol showed the best inhibitory effect on biofilm formation of P. fluorescens (37.61%) and E. carotovora (47.24%) respectively. Hexanal as QS inhibitor for biofilm formation was first reported, with inhibition ratio of 25.86% and 20.18%, which was confirmed by scanning electron and fluorescence microscopy. Inhibition of biofilm formation by anti-QS EO components could be novel intervention strategy to enhance safety and quality of vegetables in the food industry.

研究了亚MIC时10种精油（EO）成分对蔬菜变质欧文氏假单胞菌和荧光假单胞菌生物膜形成的影响。紫罗兰色杆菌CV026（CV026）被选为群体感应（QS）生物传感器细菌。确定游泳，成群，抽搐运动，胞外多糖（EPS）的产生和生物膜的形成。所有测试的EO组分均显示出对CV026，胡萝卜杆菌和荧光假单胞菌的QS抑制。生物膜被所有EO成分抑制，包括抑制其运动性和EPS生成。水杨酸和百里香酚对荧光假单胞菌的生物膜形成表现出最佳的抑制作用（37.61％），而胡萝卜肠杆菌（47.24％）分别。首次报道了己醛作为生物膜形成的QS抑制剂，其抑制率分别为25.86％和20.18％，这已通过扫描电子和荧光显微镜得到了证实。抗QS EO成分抑制生物膜形成可能是提高食品工业中蔬菜安全性和质量的新型干预策略。