Coconut testa, a brown skin covering of a coconut endosperm, is a rich source of phenolics. It is one of the by-products obtained in the coconut processing industries and currently underutilized despite being rich in phenolic compounds. A study was conducted to identify the suitable solvent system for maximum extractability of total phenolic content (TPC) and total flavonoid content (TFC) with antioxidant potential from the testa. Individual phenolic acids and flavonoids of different solvent extracts were also determined using UPLC-H class coupled with TQD-MS/MS. The TPC and TFC ranged from 4.9 to 167 mg GAE/g and 8.84–115 mg QE/g defatted testa, respectively; significant differences were observed for various solvent systems studied. Acidification of solvents significantly increased the extractability of TPC and reduced the extractability of TFC. A total of 28 phenolics comprising 16 phenolic acids and 12 flavonoids were identified. Protocatechuic acid, p-coumaric acid and ferulic acid are the major phenolic acids identified whereas, catechin, apigenin and kaempferol are the major flavonoids identified. In summary, this study proved that coconut testa is a natural source of multiple phenolics acids and flavonoids with potent antioxidant capacity, and it can be used as a natural source of antioxidants.

椰子胚乳是椰子胚乳的棕色皮肤覆盖物，是酚类的丰富来源。它是在椰子加工工业中获得的副产物之一，尽管富含酚类化合物，但目前仍未得到充分利用。进行了一项研究，以确定合适的溶剂系统，以最大的可萃取性获得总酚含量（TPC）和总黄酮含量（TFC）以及来自睾丸的抗氧化剂。还使用UPLC-H级与TQD-MS / MS联用测定了不同溶剂提取物的各个酚酸和类黄酮。TPC和TFC范围分别为4.9至167 mg GAE / g和8.84–115 mg QE / g脱脂睾丸。对于所研究的各种溶剂系统，观察到了显着差异。溶剂酸化显着提高了TPC的可萃取性，并降低了TFC的可萃取性。总共鉴定出28种酚类化合物，其中包括16种酚酸和12种类黄酮。原儿茶酸对-香豆酸和阿魏酸是鉴定出的主要酚酸，而儿茶素，芹菜素和山emp酚是鉴定出的主要类黄酮。总而言之，这项研究证明椰子睾丸是多种酚酸和类黄酮的天然来源，具有强大的抗氧化能力，并且可以用作天然抗氧化剂。