A novel Schiff base derivative of pyridoxal for the optical sensing of Zn2+ and cysteine†,Photochemical & Photobiological Sciences

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A novel Schiff base derivative of pyridoxal for the optical sensing of Zn2+ and cysteine†
Photochemical & Photobiological Sciences ( IF 2.7 ) Pub Date : 2018-02-13 00:00:00 , DOI: 10.1039/c7pp00391a
Thangaraj Anand _{1,

2,

3,

4} , Ashok S. K. Kumar _{4,

5,

6,

7,

8} , Suban K. Sahoo _{1,

2,

3,

4}

Affiliation

An easy to prepare novel vitamin B₆ cofactor derivative 3-hydroxy-N′-((3 hydroxy-5-(hydroxymethyl)-2-methylpyridin-4-yl)methylene)-2-naphthohydrazide (NPY) was synthesized by a one pot condensation reaction of pyridoxal with 3-hydroxy-2-naphthoic hydrazide and applied for the optical detection of Zn²⁺ and cysteine in the aqueous DMSO medium. The addition of Zn²⁺ ions leads to a selective blue-shift in the fluorescence emission spectrum of NPY from 530 nm to 475 nm, which allowed ratiometric detection of Zn²⁺ ions down to 8.73 × 10⁻⁷ M without any interference from other tested metal ions. This system was also successfully applied to detect intracellular Zn²⁺ ions in live HeLa cells. Further, when the in situ generated NPY·Zn²⁺ complex was interacted with various amino acids, the addition of cysteine resulted in an instantaneous colour change from light yellow to colourless and the absorbance at 435 nm of the complex was quenched selectively. Also, the fluorescence of the NPY·Zn²⁺ complex was quenched, which allowed the detection of cysteine down to 6.63 × 10⁻⁷ M.

中文翻译：

吡ido醛的新型席夫碱衍生物，用于光学检测Zn ²⁺和半胱氨酸†

一种易于制备的新型维生素B ₆辅因子衍生物3-羟基-N '-（（（3羟基-5-（羟甲基）-2-甲基吡啶-4-基）亚甲基）-2-萘甲酰肼（NPY）吡ido醛与3-羟基-2-萘酰肼的釜缩反应，用于光学检测DMSO介质中的Zn ²⁺和半胱氨酸。Zn ²⁺离子的添加导致NPY荧光发射光谱从530 nm到475 nm发生选择性蓝移，从而允许比例检测低至8.73×10 ^-7的Zn ²⁺离子。M，不受其他测试金属离子的干扰。该系统还成功地用于检测活HeLa细胞中的细胞内Zn ²⁺离子。此外，当原位产生的NPY ·Zn ²⁺复合物与各种氨基酸相互作用时，半胱氨酸的添加导致瞬时颜色从浅黄色变为无色，并且该复合物在435 nm处的吸光度被选择性淬灭。此外，NPY ·Zn ²⁺络合物的荧光被淬灭，从而可检测到低至6.63×10 ^-7 M的半胱氨酸。

更新日期：2018-02-13

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