In recent years, mass spectrometry (MS) has been establishing its role in the development of analytical methods for multiple allergen detection, but most analyses are being carried out on low-resolution mass spectrometers such as triple quadrupole or ion traps. In this investigation, performance provided by a high resolution (HR) hybrid quadrupole-Orbitrap™ MS platform for the multiple allergens detection in processed food matrix is presented. In particular, three different acquisition modes were compared: full-MS, targeted-selected ion monitoring with data-dependent fragmentation (t-SIM/dd2), and parallel reaction monitoring. In order to challenge the HR-MS platform, the sample preparation was kept as simple as possible, limited to a 30-min ultrasound-aided protein extraction followed by clean-up with disposable size exclusion cartridges. Selected peptide markers tracing for five allergenic ingredients namely skim milk, whole egg, soy flour, ground hazelnut, and ground peanut were monitored in home-made cookies chosen as model processed matrix. Timed t-SIM/dd2 was found the best choice as a good compromise between sensitivity and accuracy, accomplishing the detection of 17 peptides originating from the five allergens in the same run. The optimized method was validated in-house through the evaluation of matrix and processing effects, recoveries, and precision. The selected quantitative markers for each allergenic ingredient provided quantification of 60–100 μg_ingred/g allergenic ingredient/matrix in incurred cookies.

中文翻译：

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内部验证的高分辨率质谱法基于Orbitrap的方法在加工的模型食品中进行多种过敏原检测

近年来，质谱（MS）在多种过敏原检测分析方法的开发中已经确立了自己的作用，但是大多数分析是在低分辨率质谱仪（例如三重四极杆或离子阱）上进行的。在这项调查中，展示了高分辨率（HR）混合四极杆-Orbitrap™MS平台为加工食品基质中的多种过敏原检测提供的性能。尤其是，比较了三种不同的采集模式：全质谱，具有数据依赖性碎片的目标选择离子监测（t-SIM / dd2）和平行反应监测。为了挑战HR-MS平台，样品制备应保持尽可能简单，仅限于30分钟的超声辅助蛋白质提取，然后用一次性尺寸排阻盒进行清理。在选择作为模型处理基质的自制饼干中，对追踪到的五种致敏成分（脱脂牛奶，全蛋，大豆粉，榛子和花生粉）的选定肽标记进行了监测。定时t-SIM / dd2被发现是最佳选择，因为它是灵敏度和准确性之间的良好折衷，在同一运行中完成了对来自五种过敏原的17种肽的检测。通过评估基质和加工效果，回收率和精密度，对优化方法进行了内部验证。为每种致敏成分选择的定量标记提供了60–100μg的定量在产生的饼干中_加入/ g变应原性成分/基质。