In this experimental study, we developed lateral flow immunoassay strips for the detection of Escherichia coli. For labelling, gold nanoparticles were prepared and modified with a Raman label. As a result of this, not only colorimetric responses but also Raman responses were obtained from the same strips. The color and Raman intensities were employed for the quantitative determination of Escherichia coli. The employment of two different detection methods strengthens the quantitative determination of Escherichia coli by lateral flow immunoassay, which is mostly used for qualitative detection or sometimes the semi-quantitative determination of samples. After careful optimization and validation steps, the developed strips allowed the highly sensitive determination of Escherichia coli in water and urine samples.

中文翻译：

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用于尿液中大肠杆菌计数的纸基侧向免疫测定法†

在这项实验研究中，我们开发了用于检测大肠杆菌的侧向流免疫检测条。为了标记，制备了金纳米颗粒并用拉曼标记修饰。结果，从相同的试纸中不仅获得了比色响应，而且还获得了拉曼响应。用颜色和拉曼强度进行大肠杆菌的定量测定。两种不同检测方法的使用加强了大肠杆菌的定量测定通过侧向流免疫测定，主要用于定性检测或有时半定量测定样品。经过仔细的优化和验证步骤后，开发的试纸可以高度灵敏地测定水和尿液样品中的大肠杆菌。