Advancement in high-throughput screening methods of novel therapeutic proteins for early stage research and development, specifically mAbs, have given mid-scale (milligram to gram scale) purification groups access to more of these molecules. The available purification technologies built to support mid-scale production was not efficient or versatile enough to keep up with this surge. To remedy this problem, we have designed and built a custom instrument using an ÄKTA Pure. This system enables parallel processing up to 5 samples and has the versatility to perform 1- to 3-step purification processes in a single queue. Furthermore, a unique purification scheme can be selected for each of the five samples in the queue. Overall processing time has reduced by 83% compared to manual, non-parallel load methods. Here, we describe our novel approach and demonstrate the flexibility, speed and efficiency of the instrument.

用于早期研究和开发的新型治疗性蛋白质（特别是mAb）的高通量筛选方法的发展已使中等规模（毫克级至克级）纯化组可以使用更多此类分子。为支持中型生产而构建的可用净化技术效率或多功能性不足以跟上这一浪潮。为了解决这个问题，我们使用ÄKTAPure设计和制造了一种定制仪器。该系统能够并行处理多达5个样品，并且具有在单个队列中执行1到3步纯化过程的多功能性。此外，可以为队列中的五个样本中的每个样本选择唯一的纯化方案。与手动，非并行加载方法相比，总处理时间减少了83％。这里，