In Situ Construction of Protein-Based Semisynthetic Biosensors,ACS Sensors

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In Situ Construction of Protein-Based Semisynthetic Biosensors
ACS Sensors ( IF 8.2 ) Pub Date : 2018-02-12 00:00:00 , DOI: 10.1021/acssensors.7b00894
Tsuyoshi Ueda ₁ , Tomonori Tamura ₁ , Itaru Hamachi _{1,

2}

Affiliation

Chemically constructed biosensors consisting of a protein scaffold and an artificial small molecule have recently been recognized as attractive analytical tools for the specific detection and real-time monitoring of various biological substances or events in cells. Conventionally, such semisynthetic biosensors have been prepared in test tubes and then introduced into cells using invasive methods. With the impressive advances seen in bioorthogonal protein conjugation methodologies, however, it is now becoming feasible to directly construct semisynthetic biosensors in living cells, providing unprecedented tools for life-science research. We discuss here recent efforts regarding the in situ construction of protein-based semisynthetic biosensors and highlight their uses in the visualization and quantification of biomolecules and events in multimolecular and crowded cellular systems.

中文翻译：

基于蛋白质的半合成生物传感器的原位构建

最近，由蛋白质支架和人工小分子组成的化学构造生物传感器已被公认为是对各种生物物质或细胞中的事件进行特异性检测和实时监控的有吸引力的分析工具。常规地，已经在试管中制备了这种半合成生物传感器，然后使用侵入性方法将其引入细胞中。然而，随着生物正交蛋白偶联方法的惊人进步，直接在活细胞中构建半合成生物传感器成为可行的方法，为生命科学研究提供了前所未有的工具。我们在这里讨论有关原位的最新努力基于蛋白质的半合成生物传感器的构建，并着重介绍了它们在多分子和拥挤细胞系统中生物分子和事件的可视化和量化中的用途。

更新日期：2018-02-12

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