A novel stationary phase derived from a sulfonated chitooligosaccharide (COS) was prepared and evaluated in hydrophilic interaction liquid chromatography (HILIC) mode. Fourier-transform infrared spectroscopy (FT-IR), elemental analysis (EA), and thermogravimetric analysis (TGA) supported the successful immobilization of the obtained material. The effects of various parameters including the composition of the mobile phase, buffer concentration, buffer pH, column temperature, and stability on the retention behavior of the stationary phase were investigated by using nucleosides and bases. Two empirical models, partitioning and adsorption, were used to describe the separation mechanism of the stationary phase. The corresponding column could also successfully separate other polar and hydrophilic compounds such as water-soluble vitamins, salicylic acid related compounds, and melamine. The newly synthesized stationary phase displayed potential for application in HILIC mode separation.

中文翻译：

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磺化壳寡糖改性的硅胶材料，用于亲水相互作用液相色谱法及其色谱评估†

制备了一种衍生自磺化壳寡糖（COS）的新型固定相，并在亲水相互作用液相色谱（HILIC）模式下进行了评估。傅里叶变换红外光谱（FT-IR），元素分析（EA）和热重分析（TGA）支持成功固定所获得的材料。使用核苷和碱基研究了各种参数，包括流动相的组成，缓冲液浓度，缓冲液pH，柱温和稳定性对固定相保留行为的影响。分配和吸附这两个经验模型被用来描述固定相的分离机理。相应的色谱柱还可以成功分离其他极性和亲水性化合物，例如水溶性维生素，水杨酸相关化合物和三聚氰胺。新合成的固定相显示了在HILIC模式分离中应用的潜力。