To quickly determine the amounts of kirenol, darutoside and darutigenol in Herba Siegesbeckiae using HPLC, the efficiency of sample cleanup with different solid phase extraction methods was systematically investigated. The results showed that a polymer based strong anion exchange (P-SAX, 200 mg/6 mL) sorbent could separate and enrich diterpenoids providing rapid determination without impurity interference. In this paper a new HPLC-UV method using a core–shell column (Boltimate C18 3.0 × 100 mm, 2.7 μm) for the quantitative determination of three diterpenoids was developed. Under optimum experimental conditions, P-SAX pretreatment with a core–shell analytical column exhibited satisfactory results in the determination of diterpenoids in Herba Siegesbeckiae with recovery rates of 95–102%, relative standard deviations below 1.1%, a limit of quantitation as low as 0.1 6 μg mL⁻¹, and a short analytical runtime of 8 min. Finally, the proposed method was successfully applied in the profiling of diterpenoids in Herba Siegesbeckiae from different regions.

中文翻译：

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固相萃取，然后使用核-壳颗粒色谱柱进行HPLC-UV快速快速检测鼠尾草中的三种二萜

为了使用HPLC快速测定药草中的基里诺尔，丁香糖苷和丁香酚的含量，系统地研究了采用不同固相萃取方法净化样品的效率。结果表明，基于聚合物的强阴离子交换吸附剂（P-SAX，200 mg / 6 mL）可以分离并富集二萜类化合物，可快速测定而无杂质干扰。在本文中，开发了一种新的HPLC-UV方法，该方法使用核壳色谱柱（Boltimate C18 3.0×100 mm，2.7μm）对三种二萜类化合物进行定量测定。在最佳实验条件下，用核-壳分析柱进行的P-SAX预处理在鼠李草中二萜类化合物的测定中显示出令人满意的结果，回收率为95–102％，相对标准偏差低于1.1％，^-1，并且分析运行时间很短，为8分钟。最终，该方法成功地应用于了不同地区广Herb香中二萜类化合物的谱图分析。