Galactooligosaccharides (GOS), recognised prebiotic, can be industrially produced from lactose and commercial β-galactosidase (β-gal) from Kluyveromyces lactis. Residual lactose and glucose limit GOS applications. To handle this problem, a multienzymatic system, with β-gal and glucose oxidase (Gox), was proposed to reduce glucose content in reaction media through its oxidation to gluconic acid (GA). Besides, ultrasound (US) horn effect over the multienzymatic system to produce GOS and GA has been evaluated. A production over 40% of GOS was found in all treatments after the first h of reaction. However, glucose consumption and GA production was significantly higher (P<0.05) for sequential reaction assisted by US, obtaining the best production of GOS (49%) and GA (28%) after 2 h of reaction. The conformational and residual activity changes of enzymes under US conditions were also evaluated, Gox being positively affected whereas in β-gal hardly any change was found.

公认的益生元低聚半乳糖（GOS）可以从乳糖工业生产，商业上可以从乳酸克鲁维酵母（Kluyveromyces lactis）生产β-半乳糖苷酶（β-gal）。。残留的乳糖和葡萄糖限制了GOS的应用。为了解决该问题，提出了一种具有β-gal和葡萄糖氧化酶（Gox）的多酶系统，以通过将其氧化成葡萄糖酸（GA）来减少反应介质中的葡萄糖含量。此外，已经评估了在多酶系统上产生GOS和GA的超声角效应。在反应的第一小时后，在所有处理中均发现了超过40％的GOS产生。然而，在US辅助下的顺序反应，葡萄糖消耗和GA产生显着更高（P <0.05），反应2小时后获得最佳的GOS（49％）和GA（28％）产生。还评估了在美国条件下酶的构象和残余活性变化，Gox受到积极影响，而在β-gal中几乎未发现任何变化。